caa a22 4500 475773810 CHVBK 20180406123625.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1023/A:1007126332651 doi (NATIONALLICENCE)springer-10.1023/A:1007126332651 Immunohistochemical detection of retinoic acid receptorα in prostate carcinoma: Correlation with proliferative activity and tumor grade [Elektronische Daten] [K. Gyftopoulos, P. Perimenis, G. Sotiropoulou-Bonikou, G. Sakellaropoulos, I. Varakis, G.A. Barbalias] Background: The use of retinoids as differentiation therapy is a novel approach to prostate cancer. Retinoids act via their own nuclear receptors, RARs and RXRs, modulating gene activity, cell growth and differentiation. This study provides new data on the content and cellular distribution of RARα protein in prostate cancer specimens, in correlation to tumor grade and proliferative activity. Material and methods: A total of 84 cases of primary prostate carcinoma, divided into 3 subgroups according to tumor grade, were immunohistochemically evaluated for retinoic acid receptor-α (RARα) and Ki67 using the streptavidin/peroxidase method on formalin fixed, paraffin embedded tissues. Results: RARα positivity was detected in all cases of prostatic carcinoma, with a more profound expression in well differentiated cancers. A statistically significant correlation between RARα staining and tumor grade was found (ANOVA, p < 0.031). Ki67 immunoreactivity was present in 35.7% of cases, but no correlation with tumor grade was found. When RARα staining was correlated with Ki67 positivity, a statistically significant correlation was present (unpaired t-test, p < 0.003). Conclusions: These findings indicate that RARα expression is correlated to some extent with tumor grade and its presence is more profound in highly proliferative tumors. Further studies are needed to establish the possible clinical value of the immunohistochemical evaluation of RARα content in tumor specimens. Kluwer Academic Publishers, 2000 Immunohistochemistry nationallicence Ki67 nationallicence Prostate carcinoma nationallicence Retinoic acid receptor-α nationallicence Gyftopoulos K. Department of Urology, School of Medicine, University of Patras, Patras, Greece aut Perimenis P. Department of Urology, School of Medicine, University of Patras, Patras, Greece aut Sotiropoulou-Bonikou G. Department of Anatomy, School of Medicine, University of Patras, Patras, Greece aut Sakellaropoulos G. Department of Medical Physics, School of Medicine, University of Patras, Patras, Greece aut Varakis I. Department of Anatomy, School of Medicine, University of Patras, Patras, Greece aut Barbalias G.A. Department of Urology, School of Medicine, University of Patras, Patras, Greece aut International Urology and Nephrology Kluwer Academic Publishers 32/2(2000-06-01), 263-269 0301-1623 32:2<263 2000 32 11255 https://doi.org/10.1023/A:1007126332651 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007126332651 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gyftopoulos K. Department of Urology, School of Medicine, University of Patras, Patras, Greece aut NATIONALLICENCE 700 1- Perimenis P. Department of Urology, School of Medicine, University of Patras, Patras, Greece aut NATIONALLICENCE 700 1- Sotiropoulou-Bonikou G. Department of Anatomy, School of Medicine, University of Patras, Patras, Greece aut NATIONALLICENCE 700 1- Sakellaropoulos G. Department of Medical Physics, School of Medicine, University of Patras, Patras, Greece aut NATIONALLICENCE 700 1- Varakis I. Department of Anatomy, School of Medicine, University of Patras, Patras, Greece aut NATIONALLICENCE 700 1- Barbalias G.A. Department of Urology, School of Medicine, University of Patras, Patras, Greece aut NATIONALLICENCE 773 0- International Urology and Nephrology Kluwer Academic Publishers 32/2(2000-06-01), 263-269 0301-1623 32:2<263 2000 32 11255 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer