caa a22 4500 475773977 CHVBK 20180406123625.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s004180000162 doi (NATIONALLICENCE)springer-10.1007/s004180000162 Immunohistochemical detection of cathepsinD, K, and L in the process of endochondral ossification in the human [Elektronische Daten] [T. Nakase, M. Kaneko, T. Tomita, A. Myoui, K. Ariga, K. Sugamoto, Y. Uchiyama, T. Ochi, H. Yoshikawa] Abstract.: CathepsinsD, K, and L were immunolocalized in tissue undergoing endochondral ossification in the human. CathepsinsD, K, and L were localized in osteoclasts and chondroclasts attached to bone matrix and cartilage matrix, respectively. CathepsinsD and L were immunostained in chondrocytes. Immunolocalization of cathepsinD was limited to hypertrophic chondrocytes adjacent to the osteochondral junction. In contrast, cathepsinL was immunolocalized in both proliferating and hypertrophic chondrocytes. In the bone marrow space, cathepsinsD, K, and L were localized in multinucleated cells. CathepsinD was diffusely detected in mononuclear bone marrow cells which were negative for cathepsinsK and L. The present findings indicated that cathepsinsK, D, and L were associated with the process of endochondral ossification in the human, and suggested that these cathepsins share roles in bone and cartilage turnover in the human. Springer-Verlag, 2000 Endochondral ossification CathepsinD CathepsinK CathepsinL Immunohistochemistry nationallicence Nakase T. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut Kaneko M. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut Tomita T. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut Myoui A. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut Ariga K. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut Sugamoto K. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut Uchiyama Y. Department of First Anatomy, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut Ochi T. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut Yoshikawa H. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut https://doi.org/10.1007/s004180000162 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000162 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakase T. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut NATIONALLICENCE 700 1- Kaneko M. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut NATIONALLICENCE 700 1- Tomita T. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut NATIONALLICENCE 700 1- Myoui A. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut NATIONALLICENCE 700 1- Ariga K. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut NATIONALLICENCE 700 1- Sugamoto K. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut NATIONALLICENCE 700 1- Uchiyama Y. Department of First Anatomy, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut NATIONALLICENCE 700 1- Ochi T. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut NATIONALLICENCE 700 1- Yoshikawa H. Department of Orthopaedic Surgery, Osaka University Medical School, 2-2, Yamadaoka, Suita, 565-0871, Japan aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer