caa a22 4500 475774035 CHVBK 20180406123625.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s004180000168 doi (NATIONALLICENCE)springer-10.1007/s004180000168 Immunolocalization of calcineurin and FKBP12, the FK506-binding protein, in Hassall's corpuscles of human thymus and epidermis [Elektronische Daten] [Hajime Nishio, Kiyoshi Matsui, Hiroko Tsuji, Akiyoshi Tamura, Koichi Suzuki] Abstract.: Calcineurin, a Ca2+/calmodulin-dependent protein phosphatase, plays an important role in various physiological functions including Tcell activation. This enzyme is a target molecule for the immunosuppressants, cyclosporinA and FK506. In the present study, we investigated immunohistochemical localization of calcineurin and FKBP12, an FK506-binding protein, in human thymus and epidermis. The catalytic subunit (calcineurinA) of calcineurin was abundantly expressed in Hassall's corpuscles which were localized in the thymic medulla and represented the terminal stages of thymic medullary epithelium. The regulatory subunit (calcineurinB) of calcineurin was also expressed in high amounts in Hassall's corpuscles. In the epidermis, which shows similarities to Hassall's corpuscles, both subunits were also abundantly expressed, and their expression increased with the differentiation of keratinocytes. FKBP12 was observed to be expressed abundantly, both in Hassall's corpuscles and the entire epidermis. These findings suggest that the differentiated forms of the two cell types, which are the thymic medullary epithelial cell and the keratinocyte, are the target for pharmacological actions of FK506. Springer-Verlag, 2000 Calcineurin Thymus Hassall's corpuscles Epidermis Immunohistochemistry nationallicence Nishio Hajime Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut Matsui Kiyoshi Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut Tsuji Hiroko Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut Tamura Akiyoshi Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut Suzuki Koichi Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut https://doi.org/10.1007/s004180000168 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000168 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishio Hajime Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut NATIONALLICENCE 700 1- Matsui Kiyoshi Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut NATIONALLICENCE 700 1- Tsuji Hiroko Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut NATIONALLICENCE 700 1- Tamura Akiyoshi Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut NATIONALLICENCE 700 1- Suzuki Koichi Department of Legal Medicine, Osaka Medical College, 2-7 Daigaku, Takatsuki, 569-8686, Japan aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer