caa a22 4500 475774051 CHVBK 20180406123625.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004180000228 doi (NATIONALLICENCE)springer-10.1007/s004180000228 Localization of hepatocyte growth factor activator inhibitor type1 in Langhans' cells of human placenta [Elektronische Daten] [Hiroaki Kataoka, Jing-Yan Meng, Hiroshi Itoh, Ryouichi Hamasuna, Takeshi Shimomura, Tatsuo Suganuma, Masashi Koono] Abstract.: Activation of hepatocyte growth factor (HGF) is a crucial limiting step in HGF-induced signaling pathway. The HGF activator inhibitor type1 (HAI-1) was identified as a potent inhibitor of HGF activator (HGFA), a serine proteinase that is responsible for the activation of HGF in vivo. HAI-1 is an integral membrane Kunitz-type serine proteinase inhibitor, and its mRNA has been reported to be most abundant in the placenta. In this report, specific antibody to HAI-1 was used in an immunohistochemical procedure to determine the localization of HAI-1 in human placenta. HAI-1 was expressed in cytotrophoblasts (Langhans' cells) of the double-layered trophoblastic epithelium of chorionic villi tissue, and syncytiotrophoblasts were almost negative. On the other hand, extravillous trophoblasts of cytotrophoblastic columns showed markedly decreased immunoreactivity, and those infiltrating into the superficial decidua membrane of early placenta were hardly stainable. The amnionic epithelial cells were also immunostained intensely. The presence of HAI-1 mRNA was also confirmed in a cultured human cytotrophoblastic cell line. In addition to HAI-1, low but distinct expression of HGFA mRNA was observed in the placenta tissue and cultured cytotrophoblasts by using a sensitive RT-PCR method. Since HGF plays an essential role in the placenta development, expression of HAI-1 and HGFA may have an important regulatory role in the placenta. The localization of HAI-1 in the proliferating trophoblastic stem cells (Langhans' cells), but not in syncytiotrophoblasts and extravillous trophoblasts, suggest a possible role of HAI-1 in the proliferation of trophoblasts. Springer-Verlag, 2000 Hepatocyte growth factor (HGF) HGF activator inhibitor type1 HGF activator Placenta Trophoblast nationallicence Kataoka Hiroaki Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut Meng Jing-Yan Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut Itoh Hiroshi Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut Hamasuna Ryouichi Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut Shimomura Takeshi Research Center, Mitsubishi-Tokyo Pharmaceuticals, Yokohama, Japan aut Suganuma Tatsuo Second Department of Anatomy, Miyazaki Medical College, Miyazaki, Japan aut Koono Masashi Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut https://doi.org/10.1007/s004180000228 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000228 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kataoka Hiroaki Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut NATIONALLICENCE 700 1- Meng Jing-Yan Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut NATIONALLICENCE 700 1- Itoh Hiroshi Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut NATIONALLICENCE 700 1- Hamasuna Ryouichi Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut NATIONALLICENCE 700 1- Shimomura Takeshi Research Center, Mitsubishi-Tokyo Pharmaceuticals, Yokohama, Japan aut NATIONALLICENCE 700 1- Suganuma Tatsuo Second Department of Anatomy, Miyazaki Medical College, Miyazaki, Japan aut NATIONALLICENCE 700 1- Koono Masashi Second Department of Pathology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer