caa a22 4500 475774094 CHVBK 20180406123625.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004180000211 doi (NATIONALLICENCE)springer-10.1007/s004180000211 Age-related appearance of tubular aggregates in the skeletal muscle of almost all male inbred mice [Elektronische Daten] [Onnik Agbulut, Josette Destombes, Danièle Thiesson, Gillian Butler-Browne] Abstract.: Tubular aggregates (TAs) which have been recently observed in a few mouse myopathies are identical to those described in human diseases. In this study we show that TAs are also found in the skeletal muscle of almost all normal inbred mice strains. In these inbred strains of mice the presence of TAs is shown to be related to both age and sex. Nine different muscles were stained with the modified Gomori trichrome method to reveal the general morphology of the muscles. Anti-SERCA1 ATPase was used to confirm that the TAs were in fact accumulations of sarcoplasmic reticulum and anti-MyHCIIB to demonstrate that these accumulations were found exclusively in the typeIIB muscle fibers. An ultrastructural study confirmed the observations revealed by light microscopy that the TAs were derived from the sarcoplasmic reticulum. TAs were never observed in female inbred mice and were only found in typeIIB glycolytic muscle fibers of male inbred mice. Therefore when analyzing the effect of genetic knock out and knock in experiments on the muscle phenotype of transgenic mice one should be aware that the presence of these aggregates is a non-specific phenomenon induced by inbreeding. Springer-Verlag, 2000 Sarcoplasmic reticulum Inclusion IIB fiber Inbred mice nationallicence Agbulut Onnik Cytosquelette et Développement, UMR CNRS 7000, 105, bd. de l'hôpital, 75634 Paris Cedex 13, France aut Destombes Josette Cytosquelette et Développement, UMR CNRS 7000, 105, bd. de l'hôpital, 75634 Paris Cedex 13, France aut Thiesson Danièle Cytosquelette et Développement, UMR CNRS 7000, 105, bd. de l'hôpital, 75634 Paris Cedex 13, France aut Butler-Browne Gillian Cytosquelette et Développement, UMR CNRS 7000, 105, bd. de l'hôpital, 75634 Paris Cedex 13, France aut https://doi.org/10.1007/s004180000211 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000211 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Agbulut Onnik Cytosquelette et Développement, UMR CNRS 7000, 105, bd. de l'hôpital, 75634 Paris Cedex 13, France aut NATIONALLICENCE 700 1- Destombes Josette Cytosquelette et Développement, UMR CNRS 7000, 105, bd. de l'hôpital, 75634 Paris Cedex 13, France aut NATIONALLICENCE 700 1- Thiesson Danièle Cytosquelette et Développement, UMR CNRS 7000, 105, bd. de l'hôpital, 75634 Paris Cedex 13, France aut NATIONALLICENCE 700 1- Butler-Browne Gillian Cytosquelette et Développement, UMR CNRS 7000, 105, bd. de l'hôpital, 75634 Paris Cedex 13, France aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer