caa a22 4500 475774116 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004180000226 doi (NATIONALLICENCE)springer-10.1007/s004180000226 Effects of ATP on intracellular calcium dynamics of the perineurium of peripheral nerve bundles [Elektronische Daten] [Yutaka Shinohe, Tomoyuki Saino] Abstract.: Adenosine-5′-triphosphate (ATP) released from damaged cells can affect functions of adjacent cells. Injuries of peripheral tissue stimulate nerves, but effect of ATP on the nerve bundles is still speculative. Peripheral nerves are surrounded by perineurium, therefore the response of perineurium may be a first event of nerve stimulation at tissue injuries. The aim of the present study is to clarify whether the perineurium responds to ATP. To this end, we analyzed the dynamics of the intracellular calcium concentration ([Ca2+]i) of perineurial cells by confocal microscopy. ATP induced a [Ca2+]i increase of perineurial cells. Ca2+ channel blockers and removing of extracellular Ca2+, but not thapsigargin pretreatment, abolished ATP-induced [Ca2+]i dynamics. This indicated that the [Ca2+]i increase was due to an influx of extracellular Ca2+. Adenosine-5′-diphosphate also elicited an increase of [Ca2+]i, but P1 receptor agonists had few effects on [Ca2+]i dynamics. Suramin (an antagonist of P2X and P2Y receptors) totally inhibited ATP-induced [Ca2+]i dynamics, but reactive blue2 (a P2Y receptor antagonist) did not. Uridine-5′-triphosphate (a P2Y receptor agonist) induced no significant change in [Ca2+]i, but α,β-methylene ATP (a P2X receptor agonist) caused a [Ca2+]i increase. In conclusion, perineurial cells respond to extracellular ATP mainly via P2X receptors. Springer-Verlag, 2000 Perineurium Adenosine-5′-triphosphate Intracellular calcium Confocal microscopy nationallicence Shinohe Yutaka Department of Dental Anesthesiology, School of Dentistry, Iwate Medical University, Chuohdohri 1-3-27, Morioka 020-8505, Japan aut Saino Tomoyuki Department of Histology, School of Medicine, Iwate Medical University, Uchimaru 19-1, Morioka 020-8505, Japan aut https://doi.org/10.1007/s004180000226 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000226 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shinohe Yutaka Department of Dental Anesthesiology, School of Dentistry, Iwate Medical University, Chuohdohri 1-3-27, Morioka 020-8505, Japan aut NATIONALLICENCE 700 1- Saino Tomoyuki Department of Histology, School of Medicine, Iwate Medical University, Uchimaru 19-1, Morioka 020-8505, Japan aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer