caa a22 4500 475774132 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004180000217 doi (NATIONALLICENCE)springer-10.1007/s004180000217 Signal enhancement at the electron microscopic level using Nanogold and gold-based autometallography [Elektronische Daten] [Klara Weipoltshammer, Christian Schöfer, Marlene Almeder, Franz Wachtler] Abstract.: Immunoelectron microscopy using ultrasmall gold markers is a very sensitive method to detect molecules at high resolution. In order to discriminate the gold particles in the electron microscope, enlargement of gold particles is necessary. So far, mostly silver ions were used for deposition onto the surface of gold grains. In our study, we tested the selective deposition of gold instead of silver ions to enlarge gold particles. This was performed following immunogold detection of DNA at the surface of ultrathin sections embedded in the acrylic resin LRWhite (postembedding approach). Morphometric analysis of the distribution of DNA in human spermatocytes revealed that the method offers very good specificity and sensitivity and therefore is a good alternative to the use of silver for signal enhancement. This technique was also applied to the detection of ribosomal genes in human testis at the electron microscopic level by in situ hybridization. Ribosomal genes were localized in peri- and intranucleolar chromatin as well as in the dense fibrillar component of nucleoli. Springer-Verlag, 2000 Silver enhancement Ultrastructure Immunohistochemistry Immunoelectron microscopy Nucleolus nationallicence Weipoltshammer Klara Institute for Histology and Embryology, University Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria aut Schöfer Christian Institute for Histology and Embryology, University Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria aut Almeder Marlene Institute for Histology and Embryology, University Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria aut Wachtler Franz Institute for Histology and Embryology, University Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria aut https://doi.org/10.1007/s004180000217 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000217 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weipoltshammer Klara Institute for Histology and Embryology, University Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria aut NATIONALLICENCE 700 1- Schöfer Christian Institute for Histology and Embryology, University Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria aut NATIONALLICENCE 700 1- Almeder Marlene Institute for Histology and Embryology, University Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria aut NATIONALLICENCE 700 1- Wachtler Franz Institute for Histology and Embryology, University Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer