caa a22 4500 475774140 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004180000186 doi (NATIONALLICENCE)springer-10.1007/s004180000186 Differential localization of organic cation transporters rOCT1 and rOCT2 in the basolateral membrane of rat kidney proximal tubules [Elektronische Daten] [Minako Sugawara-Yokoo, Yumiko Urakami, Haruko Koyama, Keiko Fujikura, Satohiro Masuda, Hideyuki Saito, Takuji Naruse, Ken-ichi Inui, Kuniaki Takata] Abstract.: Organic cation transporters play an important role in the secretion of cationic drugs as well as endogenous cationic metabolites in the renal tubules. Immunoblotting showed the presence of organic cation transporter proteins, rOCT1 and rOCT2, in the rat kidney. By immunofluorescence microscopy, rOCT1 was shown to be concentrated in the proximal tubules in the renal cortex. rOCT2, on the other hand, was rich in the proximal tubules in the outer stripe of the outer medulla. Confocal microscopy revealed that both rOCT1 and rOCT2 were localized to the basolateral membranes of these tubule cells. These findings directly show that rOCT1 and rOCT2 are basolateral membrane proteins and are differentially distributed along the proximal tubules. Springer-Verlag, 2000 Organic cation transporter rOCT1 rOCT2 Kidney Proximal tubule nationallicence Sugawara-Yokoo Minako Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan aut Urakami Yumiko Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan aut Koyama Haruko Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan aut Fujikura Keiko Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan aut Masuda Satohiro Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan aut Saito Hideyuki Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan aut Naruse Takuji The Third Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Gunma 371-8511, Japan aut Inui Ken-ichi Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan aut Takata Kuniaki Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan aut https://doi.org/10.1007/s004180000186 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000186 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugawara-Yokoo Minako Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan aut NATIONALLICENCE 700 1- Urakami Yumiko Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan aut NATIONALLICENCE 700 1- Koyama Haruko Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan aut NATIONALLICENCE 700 1- Fujikura Keiko Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan aut NATIONALLICENCE 700 1- Masuda Satohiro Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan aut NATIONALLICENCE 700 1- Saito Hideyuki Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan aut NATIONALLICENCE 700 1- Naruse Takuji The Third Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Gunma 371-8511, Japan aut NATIONALLICENCE 700 1- Inui Ken-ichi Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan aut NATIONALLICENCE 700 1- Takata Kuniaki Laboratory of Molecular and Cellular Morphology, Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8512, Japan aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer