caa a22 4500 475774159 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004180000181 doi (NATIONALLICENCE)springer-10.1007/s004180000181 Expression of transmembrane carbonic anhydrase isoenzymesIX and XII in normal human pancreas and pancreatic tumours [Elektronische Daten] [Antti J. Kivelä, Seppo Parkkila, Juha Saarnio, Tuomo J. Karttunen, Jyrki Kivelä, Anna-Kaisa Parkkila, Silvia Pastoreková, Jaromir Pastorek, Abdul Waheed, William S. Sly, Hannu Rajaniemi] Abstract.: Carbonic anhydrase (CA) IX and XII are transmembrane isoenzymes which are expressed in several epithelia and overexpressed in some carcinomas. They have recently been linked to von Hippel-Lindau gene-mediated carcinogenesis in that both isoenzymes are downregulated by the product of the wild-type von Hippel-Lindau tumour suppressor gene. This paper describes the localisation of CAIX and XII in the normal human pancreas and pancreatic tumours. Both isoenzymes showed positive reaction in the basolateral plasma membrane of the normal acinar and ductal epithelia. The hyperplastic ductal epithelium in tumour specimens generally showed an increased staining for CAIX. Of 29 malignant tumours of exocrine pancreas, 10 showed moderate or strong immunoreaction for CAIX. The signal for CAXII remained weak in most malignant lesions. The present results show that both CAIX and XII are unevenly expressed in the ductal and acinar compartments of the human pancreas. The expression of these isoenzymes in a relatively low number of malignant tumour specimens suggests that they have a limited value in diagnostic evaluation of pancreatic carcinoma. However, the increased expression of CAIX in hyperplastic ductal epithelium may contribute to the pancreatic tumourigenesis. Springer-Verlag, 2000 Adenoma Cancer Carbonic anhydrase Gastrointestinal Human Immunohistochemistry Pancreas Plasma membrane nationallicence Kivelä Antti J. Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut Parkkila Seppo Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut Saarnio Juha Department of Surgery, University of Oulu, Oulu, Finland aut Karttunen Tuomo J. Department of Pathology, University of Oulu, Oulu, Finland aut Kivelä Jyrki Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut Parkkila Anna-Kaisa Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut Pastoreková Silvia Department of Molecular Biology, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic aut Pastorek Jaromir Department of Molecular Biology, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic aut Waheed Abdul Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, Missouri, USA aut Sly William S. Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, Missouri, USA aut Rajaniemi Hannu Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut https://doi.org/10.1007/s004180000181 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000181 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kivelä Antti J. Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut NATIONALLICENCE 700 1- Parkkila Seppo Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut NATIONALLICENCE 700 1- Saarnio Juha Department of Surgery, University of Oulu, Oulu, Finland aut NATIONALLICENCE 700 1- Karttunen Tuomo J. Department of Pathology, University of Oulu, Oulu, Finland aut NATIONALLICENCE 700 1- Kivelä Jyrki Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut NATIONALLICENCE 700 1- Parkkila Anna-Kaisa Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut NATIONALLICENCE 700 1- Pastoreková Silvia Department of Molecular Biology, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Pastorek Jaromir Department of Molecular Biology, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic aut NATIONALLICENCE 700 1- Waheed Abdul Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, Missouri, USA aut NATIONALLICENCE 700 1- Sly William S. Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, Missouri, USA aut NATIONALLICENCE 700 1- Rajaniemi Hannu Department of Anatomy and Cell Biology, Box 5000, University of Oulu, 90014 Oulu, Finland aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer