caa a22 4500 475774175 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004180000180 doi (NATIONALLICENCE)springer-10.1007/s004180000180 Immunolocalization of glutamic acid decarboxylase in postmortem human cerebellar cortex. A light microscopy study [Elektronische Daten] [Vincenzo Benagiano, Paolo Flace, Daniela Virgintino, Luisa Roncali, Anna Rizzi, Glauco Ambrosi] Abstract.: The distribution of glutamic acid decarboxylase (GAD), the γ-aminobutyric acid (GABA) synthesizing enzyme, was examined in the postmortem human cerebellar cortex by immunocytochemistry. The results, obtained on nervous tissues taken at autopsy and fixed within 24-36h after death, enabled the authors to precisely reveal the topographical distribution of GAD-containing neurons and axon terminals in the human cerebellar cortex. Labeled neurons, corresponding to different neuronal cytotypes of the cerebellar cortex, showed a diffuse cytoplasmic immunoreactivity in both bodies and processes. Labeled axon terminals appeared as immunoreactive puncta. The use of immunocytochemistry in the detection of GAD in the postmortem human brain greatly increases the possibility of carrying out morphological studies on the GABAergic system, both in normal and in pathological conditions. Springer-Verlag, 2000 Human Cerebellar cortex Postmortem samples Glutamic acid decarboxylase (GAD) Immunocytochemistry nationallicence Benagiano Vincenzo Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut Flace Paolo Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut Virgintino Daniela Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut Roncali Luisa Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut Rizzi Anna Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut Ambrosi Glauco Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut https://doi.org/10.1007/s004180000180 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000180 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Benagiano Vincenzo Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut NATIONALLICENCE 700 1- Flace Paolo Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut NATIONALLICENCE 700 1- Virgintino Daniela Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut NATIONALLICENCE 700 1- Roncali Luisa Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut NATIONALLICENCE 700 1- Rizzi Anna Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut NATIONALLICENCE 700 1- Ambrosi Glauco Department of Human Anatomy and Histology, University of Bari, Policlinico, Piazza Giulio Cesare, 70124 Bari, Italy aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer