caa a22 4500 47577423X CHVBK 20180406123626.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004180000167 doi (NATIONALLICENCE)springer-10.1007/s004180000167 Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic [Elektronische Daten] [Nadia A. Ameen, Elly van Donselaar, George Posthuma, Hugo de Jonge, Gwenn McLaughlin, Hans J. Geuze, Christopher Marino, Peter J. Peters] Abstract.: The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-activated chloride channel critical to intestinal anion secretion. In addition to phosphorylation, vesicle traffic regulates CFTR in some epithelial cells. Studies of cultured intestinal cells are conflicting regarding the role of cAMP-dependent vesicle traffic in regulating chloride transport. Whether CFTR is present in vesicular compartments within chloride secretory cells in the intestine is unknown and the role of cAMP-dependent vesicle insertion in regulating CFTR and intestinal fluid secretion remains unclear. The purpose of this study was to: (1) examine and quantify the subcellular distribution for CFTR in rat intestine, (2) further define the ultrastructure of the previously identified CFTR High Expresser (CHE) cell, and (3) examine the cellular distribution of CFTR following cAMP stimulation in vivo. Using the sensitive techniques of cryoimmunogold electron microscopy we identified CFTR in subapical vesicles and on the apical plasma membrane in crypt, Brunner glands, and CHE cells. cAMP stimulation in rat proximal small intestine produced a fluid secretory response and was associated with an apical redistribution of CFTR, supporting a physiologic role for cAMP-dependent CFTR vesicle insertion in regulating CFTR in the intestine. Springer-Verlag, 2000 CFTR Subcellular localization Vesicle traffic Intestine nationallicence Ameen Nadia A. Department of Pediatrics, University of Miami School of Medicine, MCCD Room 6006B, 1601 NW 12th Avenue, Miami, FL 33136, USA aut van Donselaar Elly Department of Cell Biology and Graduate School of Biomembranes, University of Utrecht, The Netherlands aut Posthuma George Department of Cell Biology and Graduate School of Biomembranes, University of Utrecht, The Netherlands aut de Jonge Hugo Department of Biochemistry, Erasmus University, Rotterdam, The Netherlands aut McLaughlin Gwenn Department of Pediatrics, University of Miami School of Medicine, MCCD Room 6006B, 1601 NW 12th Avenue, Miami, FL 33136, USA aut Geuze Hans J. Department of Cell Biology and Graduate School of Biomembranes, University of Utrecht, The Netherlands aut Marino Christopher Department of Medicine, Yale University School of Medicine, New Haven, CT, USA aut Peters Peter J. Department of Cell Biology and Graduate School of Biomembranes, University of Utrecht, The Netherlands aut https://doi.org/10.1007/s004180000167 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000167 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ameen Nadia A. Department of Pediatrics, University of Miami School of Medicine, MCCD Room 6006B, 1601 NW 12th Avenue, Miami, FL 33136, USA aut NATIONALLICENCE 700 1- van Donselaar Elly Department of Cell Biology and Graduate School of Biomembranes, University of Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Posthuma George Department of Cell Biology and Graduate School of Biomembranes, University of Utrecht, The Netherlands aut NATIONALLICENCE 700 1- de Jonge Hugo Department of Biochemistry, Erasmus University, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- McLaughlin Gwenn Department of Pediatrics, University of Miami School of Medicine, MCCD Room 6006B, 1601 NW 12th Avenue, Miami, FL 33136, USA aut NATIONALLICENCE 700 1- Geuze Hans J. Department of Cell Biology and Graduate School of Biomembranes, University of Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Marino Christopher Department of Medicine, Yale University School of Medicine, New Haven, CT, USA aut NATIONALLICENCE 700 1- Peters Peter J. Department of Cell Biology and Graduate School of Biomembranes, University of Utrecht, The Netherlands aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer