caa a22 4500 475774256 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s004180000197 doi (NATIONALLICENCE)springer-10.1007/s004180000197 The human homologue of the Aspergillus nuclear migration gene nudC is preferentially expressed in dividing cells and ciliated epithelia [Elektronische Daten] [Christopher D. Gocke, Stephen A. Osmani, Barbara A. Miller] Abstract.: We recently identified a novel human gene, HnudC, homologous to an Aspergillus nidulans gene coding for a protein crucial to nuclear migration, cell wall morphogenesis, and cell growth. While mRNA for this gene is expressed in most tissues, HNUDC protein expression is highly regulated. To provide insight into the function of this protein, we performed immunohistochemical analysis of the distribution of HNUDC in 19 different human tissues. Intense immunolabeling was observed in proliferating cells, including spermatocytes at all stages, early hematopoietic cells, cortical thymocytes, immunoblasts, and basal colonic and esophageal mucosa. Within a given tissue, cells with different proliferative capacities demonstrated different levels of HNUDC expression. HNUDC was also highly expressed in ciliated epithelia including those found in ependyma, bronchial mucosa, and fallopian tubes. Immunolabeling was moderate in several non-proliferating tissues, but little or no labeling was observed in most other tissues examined. We also demonstrated by western blotting that most cell lines express extremely high levels of HNUDC compared to their normal counterparts. While this supports a role for HnudC in cell proliferation, these data indicate that cell lines are not a reliable measure of HNUDC protein expression in normal tissues. We conclude that HNUDC is highly expressed in cell lines and the proliferating cells of normal tissues, consistent with our hypothesis that HNUDC is conserved throughout evolution for a crucial function in cell division. In addition, the high level in ciliated cells suggests an important role in ciliary motility or assembly, analogous to its role in A. nidulans nuclear movement. Springer-Verlag, 2000 NUDC Expression Immunohistochemistry Cilia Proliferation Cell line nationallicence Gocke Christopher D. Department of Pathology, University of Maryland, Room NBW78, 22 S. Greene Street, Baltimore, MD21201, USA aut Osmani Stephen A. Departments of Pathology, Pediatrics, and Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, Pennsylvania, USA aut Miller Barbara A. Departments of Pathology, Pediatrics, and Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, Pennsylvania, USA aut https://doi.org/10.1007/s004180000197 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000197 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gocke Christopher D. Department of Pathology, University of Maryland, Room NBW78, 22 S. Greene Street, Baltimore, MD21201, USA aut NATIONALLICENCE 700 1- Osmani Stephen A. Departments of Pathology, Pediatrics, and Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, Pennsylvania, USA aut NATIONALLICENCE 700 1- Miller Barbara A. Departments of Pathology, Pediatrics, and Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, Pennsylvania, USA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer