caa a22 4500 475774264 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s004180000189 doi (NATIONALLICENCE)springer-10.1007/s004180000189 Outside-in regulation of integrin clustering processes by ECM components per se and their involvement in actin cytoskeleton organization in a colon adenocarcinoma cell line [Elektronische Daten] [Stéphane Honoré, Véronique Pichard, Claude Penel, Véronique Rigot, Charles Prévôt, Jacques Marvaldi, Claudette Briand, Jean-Baptiste Rognoni] Abstract.: We investigated in a colon adenocarcinoma cell line, the exclusive role of extracellular matrix (ECM) components in the absence of soluble factors regarding the integrin clustering processes, and their implication in cell adhesion, spreading and organization of the actin cytoskeleton. Caco-2 cells were shown to express at the plasma membrane 11 integrins, some of which (e.g. α3β1, α5β1, α6β1/β4, α8β1 and αvβ1/β5/β6) were identified for the first time in this cell line. Cell adhesion and spreading processes were governed essentially by lamellipodium, the regulation of which was shown to be induced by two types of integrin clustering processes mediated by ECM proteins alone. During these phenomena, α2β1, αvβ6 and α6β1 integrins, the Caco-2 cell specific receptors of type IV collagen, fibronectin and laminin, respectively, were clustered in small focal complexes (point contacts), whereas αvβ5, the vitronectin receptor in this cell line, was aggregated in focal adhesions. The two levels of integrin clustering induced only F-actin cortical web formation organized in thin radial and/or circular filaments. We conclude thus that ECM components per se through their action on integrin clustering are involved in cell adhesion, cortical actin cytoskeleton organization and cell spreading. Springer-Verlag, 2000 Cell adhesion Cell spreading Integrin clustering Actin cytoskeleton Colic adenocarcinoma cell line nationallicence Honoré Stéphane UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut Pichard Véronique UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut Penel Claude Laboratoire de Cancérologie expérimentale, Faculté de Médecine Nord, Bd Pierre Dramard 13916, Marseille Cedex 20, France aut Rigot Véronique UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut Prévôt Charles UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut Marvaldi Jacques UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut Briand Claudette UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut Rognoni Jean-Baptiste UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut https://doi.org/10.1007/s004180000189 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000189 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Honoré Stéphane UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut NATIONALLICENCE 700 1- Pichard Véronique UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut NATIONALLICENCE 700 1- Penel Claude Laboratoire de Cancérologie expérimentale, Faculté de Médecine Nord, Bd Pierre Dramard 13916, Marseille Cedex 20, France aut NATIONALLICENCE 700 1- Rigot Véronique UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut NATIONALLICENCE 700 1- Prévôt Charles UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut NATIONALLICENCE 700 1- Marvaldi Jacques UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut NATIONALLICENCE 700 1- Briand Claudette UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut NATIONALLICENCE 700 1- Rognoni Jean-Baptiste UPRES-A CNRS 6032, Faculté de Pharmacie, Université Aix-Marseille I et II, 27, Bd Jean Moulin 13385, Marseille Cedex 05, France aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer