caa a22 4500 475774272 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s004180000185 doi (NATIONALLICENCE)springer-10.1007/s004180000185 Multiplex FISH and three-dimensional DNA imaging with near infrared femtosecond laser pulses [Elektronische Daten] [K. König, I. Riemann, P. Fischer, K.J. Halbhuber] Abstract.: We report on a novel technology for multicolor gene and chromosome detection as well as for three-dimensional (3D) DNA imaging by multiphoton excitation of multiple FISH fluorophores and DNA stains. Near infrared femtosecond laser pulses at 770nm were used to simultaneously excite the visible fluorescence of a wide range of FISH fluorophores, such as FITC, DAC, Cy3, Cy5, Cy5.5, rhodamine, spectrum aqua, spectrum green, spectrum orange, Jenfluor, and Texas red as well as of DNA/chromosome stains, for example Hoechst33342, DAPI, SYBR green, propidium iodide, ethidium homodimer, and Giemsa. In addition to the advantage of using only one excitation wavelength for a variety of fluorophores, multiphoton excitation provided the intrinsic possibility of 3D fluorescence imaging. The technology has been used in human genetics for the diagnosis of numerical chromosome aberrations and microdeletions. In particular, multicolor 3D images of the intranuclear localization of FISH-labeled chromosome territories in interphase nuclei of amniotic fluid cells have been obtained. Using the high light penetration depth at 770nm, optical sectioning of Hoechst33342-labeled DNA within living culture cells and within tissue of living tumor-bearing mice was performed. Springer-Verlag, 2000 Fluorescence in situ hybridization Multigene detection NIR Two photon 3D nationallicence König K. Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben7, 07743 Jena, Germany aut Riemann I. Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben7, 07743 Jena, Germany aut Fischer P. Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben7, 07743 Jena, Germany aut Halbhuber K.J. Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben7, 07743 Jena, Germany aut https://doi.org/10.1007/s004180000185 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000185 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- König K. Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben7, 07743 Jena, Germany aut NATIONALLICENCE 700 1- Riemann I. Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben7, 07743 Jena, Germany aut NATIONALLICENCE 700 1- Fischer P. Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben7, 07743 Jena, Germany aut NATIONALLICENCE 700 1- Halbhuber K.J. Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben7, 07743 Jena, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer