caa a22 4500 475774310 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s004180000202 doi (NATIONALLICENCE)springer-10.1007/s004180000202 Resistance of L132 lung cell clusters to glyoxal-induced apoptosis [Elektronische Daten] [C. Roehlecke, A.-K. Kuhnt, H. Fehrenbach, C. Werner, R.H.W. Funk, M. Kasper] Abstract.: Glyoxal is a highly reactive glycating agent involved in the formation of advanced glycation end products (AGEs) and known to induce apoptosis. AGE-mediated apoptosis may be an important mechanism of alveolar epithelial remodelling in pulmonary fibrosis. In this study, we investigated the cytotoxic effect of glyoxal on the fetal human epithelial lung cell line L132 under serum-free conditions. This type of culture, which forces the cells to grow as spheroids, also excludes effects of preformed AGEs by the reaction of glyoxal with fetal calf serum proteins. Our results showed that in cells treated with 200µM glyoxal, the intercellular contacts in spheroids were disrupted, i.e. cells became totally dissociated. Immunocytochemical analysis revealed a dose-dependent accumulation of the AGE product ε N-(carboxymethyl)lysine (CML) in cells detached from cell clusters. The loss of cell attachment was associated with decreased expression of β1-integrins and CD44 as revealed by laser scanning cytometry (LSC). Increasing concentrations of glyoxal induced an increase in the number of apoptotic cells which were identified by the immunoreactivity for active caspase-3. Remaining cell clusters showed resistance to both CML formation and apoptosis. The present findings demonstrate that cells treated with glyoxal undergo possibly anoikis, a specific mode of apoptosis caused by loss of cell adhesion. Springer-Verlag, 2000 Glyoxal AGE CML Apoptosis Pulmonary fibrosis nationallicence Roehlecke C. Department of Anatomy, Technical University Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut Kuhnt A.-K Department of Anatomy, Technical University Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut Fehrenbach H. Institute of Pathology, University Clinic "Carl Gustav Carus" at the Technical University of Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut Werner C. Institute of Polymer Research, Hohe Str. 6, 01069 Dresden, Germany aut Funk R.H.W. Department of Anatomy, Technical University Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut Kasper M. Department of Anatomy, Technical University Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut https://doi.org/10.1007/s004180000202 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000202 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Roehlecke C. Department of Anatomy, Technical University Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut NATIONALLICENCE 700 1- Kuhnt A.-K Department of Anatomy, Technical University Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut NATIONALLICENCE 700 1- Fehrenbach H. Institute of Pathology, University Clinic "Carl Gustav Carus" at the Technical University of Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut NATIONALLICENCE 700 1- Werner C. Institute of Polymer Research, Hohe Str. 6, 01069 Dresden, Germany aut NATIONALLICENCE 700 1- Funk R.H.W. Department of Anatomy, Technical University Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut NATIONALLICENCE 700 1- Kasper M. Department of Anatomy, Technical University Dresden, Fetscherstr. 74, 01307 Dresden, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer