caa a22 4500 475774329 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004180000206 doi (NATIONALLICENCE)springer-10.1007/s004180000206 Acid secretion of parietal cells is paralleled by a redistribution of NSF and α,β-SNAPs and inhibited by tetanus toxin [Elektronische Daten] [Seija Lehnardt, Gudrun Ahnert-Hilger, Hans Bigalke, Thomas Jöns] Abstract.: Stimulation of parietal cells causes fusion of intracellular tubulovesicles with the canalicular plasma membrane thereby increasing the apical membrane area up to tenfold. The presence of the SNARE proteins synaptobrevin, syntaxin1, and SNAP25 in parietal cells and their intracellular redistribution after stimulation suggest a SNARE-mediated mechanism. Here we show that NSF and α,β-SNAPs which are involved in the dissociation of the SNARE complex in neurons also occur in parietal cells exhibiting subcellular distributions similar to the ones obtained for SNARE proteins and for the H+,K+-ATPase. More importantly proteolytic cleavage of synaptobrevin by tetanus neurotoxin completely inhibits the cAMP-dependent increase of acid secretion further supporting the crucial role SNARE proteins play in parietal cells. Springer-Verlag, 2000 Parietal cell Homotypic fusion NSF α,β-SNAPs Tetanus neurotoxin Acid secretion nationallicence Lehnardt Seija Institut für Anatomie der Charité Humboldt-Universität zu Berlin, 10117 Berlin, Germany aut Ahnert-Hilger Gudrun Institut für Anatomie der Charité Humboldt-Universität zu Berlin, 10117 Berlin, Germany aut Bigalke Hans Institut für Toxikologie der Medizinischen Hochschule Hannover, 30623 Hannover, Germany aut Jöns Thomas Institut für Anatomie der Charité Humboldt-Universität zu Berlin, Philippstrasse 12, 10117 Berlin, Germany aut https://doi.org/10.1007/s004180000206 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000206 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lehnardt Seija Institut für Anatomie der Charité Humboldt-Universität zu Berlin, 10117 Berlin, Germany aut NATIONALLICENCE 700 1- Ahnert-Hilger Gudrun Institut für Anatomie der Charité Humboldt-Universität zu Berlin, 10117 Berlin, Germany aut NATIONALLICENCE 700 1- Bigalke Hans Institut für Toxikologie der Medizinischen Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 700 1- Jöns Thomas Institut für Anatomie der Charité Humboldt-Universität zu Berlin, Philippstrasse 12, 10117 Berlin, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer