caa a22 4500 475774337 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004180000196 doi (NATIONALLICENCE)springer-10.1007/s004180000196 Immunohistochemical analysis of steroid receptors and glycodelinA (PP14) in isolated glandular epithelial cells of normal human endometrium [Elektronische Daten] [Ioannis Mylonas, Runa Speer, Josef Makovitzky, Dagmar U. Richter, Volker Briese, Udo Jeschke, Klaus Friese] Abstract.: Highly purified fractions of isolated endometrial cells can be useful for investigating endometrial function. After a first collagenase digestion, normal human endometrial stromal and epithelial cells were separated by filtration. Glands were purified further by two collagenase digestion steps, filtration, differential sedimentations, and Ficoll gradient centrifugation. Epithelial cells were polyhedral and grew as islands in a whorl-like wavy pattern around glandular fragments. High cell culture purity was confirmed with the positive immunohistochemical reaction against cytokeratin7,8,18,19. Isolated human glands had a similar distribution pattern of estrogen receptor (ER) and progesterone receptor (PR) as observed in vivo, suggesting that glands have a functional hormone receptor system at the time of plating. Using a specific monoclonal antibody against glycodelinA (GdA), a characteristic cyclical expression was demonstrated during the menstrual cycle. The GdA reaction was weak in the proliferative phase, increasing significantly till the late secretory phase, suggesting a similar GdA concentration in vitro as observed in vivo glands. In conclusion, this method could be a model for studying endometrial glandular cells from different menstrual phases, endometrial cell interactions, implantation mechanisms, GdA regulation mechanisms, and pharmacological or other influences on ER and PR alteration. Springer-Verlag, 2000 Endometrium Glandular cells Estrogen receptor (ER) Progesterone receptor (PR) GlycodelinA (PP14) nationallicence Mylonas Ioannis Brown University and School of Medicine, Providence, Rhode Island, USA aut Speer Runa Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut Makovitzky Josef Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut Richter Dagmar U. Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut Briese Volker Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut Jeschke Udo Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut Friese Klaus Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut https://doi.org/10.1007/s004180000196 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000196 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mylonas Ioannis Brown University and School of Medicine, Providence, Rhode Island, USA aut NATIONALLICENCE 700 1- Speer Runa Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut NATIONALLICENCE 700 1- Makovitzky Josef Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut NATIONALLICENCE 700 1- Richter Dagmar U. Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut NATIONALLICENCE 700 1- Briese Volker Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut NATIONALLICENCE 700 1- Jeschke Udo Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut NATIONALLICENCE 700 1- Friese Klaus Department of Obstetrics and Gynaecology, University of Rostock, Doberanerstrasse 142, 18055 Rostock, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer