caa a22 4500 475774361 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004180000204 doi (NATIONALLICENCE)springer-10.1007/s004180000204 Active interaction of human A375 melanoma cells with the lymphatics in vivo [Elektronische Daten] [Maria Papoutsi, Gerhard Siemeister, Karin Weindel, Stanislav I. Tomarev, Haymo Kurz, Christoph Schächtele, Georg Martiny-Baron, Bodo Christ, Dieter Marmé, Jörg Wilting] Abstract.: We have used the avian chorioallantoic membrane (CAM) to study the interaction of tumor cells with the lymphatics in vivo. The vascular endothelial growth factor-C (VEGF-C) has been shown to be lymphangiogenic. We have therefore grown VEGF-C-expressing human A375 melanoma cells on the CAM. These tumors induced numerous lymphatics at the invasive front, and compressed or destroyed VEGFreceptor (R)-3-positive lymphatics were observed within the solid tumors. The lymphatics in the CAM and in the A375 melanomas could also be demonstrated with an antibody against Prox1, a highly specific marker of lymphatic endothelial cells. Proliferation studies revealed a BrdU labeling index of 11.6% of the lymphatic endothelial cells in the tumors and at their margins. A great number of melanoma cells invaded the lymphatics. Such interactions were not observed with VEGF-C-negative Malme 3M melanoma cells. Lymphangiogenesis was inhibited to some extent when A375 melanoma cells were transfected with cDNA encoding soluble VEGFR-3 (sflt4), and the BrdU labeling index of the lymphatics in these tumors was 3.9%. Invasion of lymphatics and growth of blood vascular capillaries were not inhibited by the transfection. Therefore, tumor-induced lymphangiogenesis seems to be dependent to some extent on VEGF-C/flt4 interactions, but invasion of lymphatics seems to be a distinct mechanism. Springer-Verlag, 2000 Lymphangiogenesis Tumor Chorioallantoic membrane VEGF-C Prox1 Lymphatic invasion Avian embryo nationallicence Papoutsi Maria Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Albertstrasse 17, 79104 Freiburg i. Br., Germany aut Siemeister Gerhard Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut Weindel Karin Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut Tomarev Stanislav I. Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institute of Health, Bethesda, Maryland 20892-2730, USA aut Kurz Haymo Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Albertstrasse 17, 79104 Freiburg i. Br., Germany aut Schächtele Christoph Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut Martiny-Baron Georg Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut Christ Bodo Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Albertstrasse 17, 79104 Freiburg i. Br., Germany aut Marmé Dieter Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut Wilting Jörg Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Albertstrasse 17, 79104 Freiburg i. Br., Germany aut https://doi.org/10.1007/s004180000204 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000204 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Papoutsi Maria Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Albertstrasse 17, 79104 Freiburg i. Br., Germany aut NATIONALLICENCE 700 1- Siemeister Gerhard Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut NATIONALLICENCE 700 1- Weindel Karin Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut NATIONALLICENCE 700 1- Tomarev Stanislav I. Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institute of Health, Bethesda, Maryland 20892-2730, USA aut NATIONALLICENCE 700 1- Kurz Haymo Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Albertstrasse 17, 79104 Freiburg i. Br., Germany aut NATIONALLICENCE 700 1- Schächtele Christoph Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut NATIONALLICENCE 700 1- Martiny-Baron Georg Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut NATIONALLICENCE 700 1- Christ Bodo Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Albertstrasse 17, 79104 Freiburg i. Br., Germany aut NATIONALLICENCE 700 1- Marmé Dieter Institut für Molekulare Medizin und Naturstofforschung der Albert-Ludwigs-Universität, Breisacherstrasse 117, 79106 Freiburg i. Br., Germany aut NATIONALLICENCE 700 1- Wilting Jörg Anatomisches Institut der Albert-Ludwigs-Universität, Lehrstuhl II, Albertstrasse 17, 79104 Freiburg i. Br., Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer