caa a22 4500 475774388 CHVBK 20180406123626.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004180000201 doi (NATIONALLICENCE)springer-10.1007/s004180000201 Heterogeneous expression of mRNA in rat liver lobules as detected by differential display [Elektronische Daten] [Gernot Gläßer, Rolf Gebhardt, Frank Gaunitz] Abstract.: Although liver hepatocytes appear to be uniform histologically, they are considerably heterogeneous with respect to their individual physiological capacities. In order to find still unknown genes that are heterogeneously expressed and with the aim of evaluating the usefulness of the differential display technique for this purpose, we performed differential displays with mRNA isolated from hepatocytes from the periportal and pericentral zone of the rat liver. In this way we identified at least two mRNAs exclusively expressed in the pericentral fraction. Sequence analysis revealed that the corresponding genes encode proteins with proline-glutamate dipeptide repeats similar to ones previously identified in rat pheochromocytoma and brain. In situ hybridization confirmed the heterogeneous distribution of the mRNA. Only one to two cell lines surrounding the terminal hepatic venules were positive, strongly resembling the heterogeneous expression of the enzyme glutamine synthetase. Our work demonstrates that the differential display method is a useful tool for the identification of genes that are differentially expressed in individual parenchymal cells. In fact, our results prove that differential display technology can be used for the identification of cellular markers for distinct subpopulations of cells in a given tissue. Springer-Verlag, 2000 Differential display Liver heterogeneity Proline-glutamate-dipeptide repeat RT-PCR nationallicence Gläßer Gernot Institut für Biochemie, Medizinische Fakultät, Universitätsklinikum Leipzig, Liebigstrasse 16, 04103 Leipzig, Germany aut Gebhardt Rolf Institut für Biochemie, Medizinische Fakultät, Universitätsklinikum Leipzig, Liebigstrasse 16, 04103 Leipzig, Germany aut Gaunitz Frank Institut für Biochemie, Medizinische Fakultät, Universitätsklinikum Leipzig, Liebigstrasse 16, 04103 Leipzig, Germany aut https://doi.org/10.1007/s004180000201 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000201 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gläßer Gernot Institut für Biochemie, Medizinische Fakultät, Universitätsklinikum Leipzig, Liebigstrasse 16, 04103 Leipzig, Germany aut NATIONALLICENCE 700 1- Gebhardt Rolf Institut für Biochemie, Medizinische Fakultät, Universitätsklinikum Leipzig, Liebigstrasse 16, 04103 Leipzig, Germany aut NATIONALLICENCE 700 1- Gaunitz Frank Institut für Biochemie, Medizinische Fakultät, Universitätsklinikum Leipzig, Liebigstrasse 16, 04103 Leipzig, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer