caa a22 4500 475774434 CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s004180000171 doi (NATIONALLICENCE)springer-10.1007/s004180000171 Vertebrate phylogeny of antigenD10: identification of a conserved foregut cell lineage [Elektronische Daten] [Simon G. Royce, Norman R. Hughes, Steve Binos, John R. Underwood, Prithi S. Bhathal] Abstract.: The monoclonal antibody 5HL-5D11-D10 to antigenD10 identifies a cell lineage that is restricted to certain tissues of the human foregut. We investigated the tissue distribution of antigenD10 in mammals, birds, reptiles, amphibians and fish by immunohistochemical staining. Tissue from human and each of ten other mammalian species showed staining of gastric mucous neck cells and glands of the cardia and antrum, Brunner's glands, peribiliary glands and periductal glands of the pancreas. Six of the mammalian species also expressed antigenD10 in mucosa of the larger bronchi, and five expressed it to varying degree in small bowel distal to the duodenum and in colon (three of these five species). Antigen was not detected in any of the three species of bird studied. Both reptiles and amphibians showed strong staining for antigenD10 in the gastric mucous neck cells and pyloric glands, and in a subpopulation of secretory cells in the oesophagus, with the amphibian also expressing antigen in some epithelial cells of the mouth and lung. Although absent from two species of bony fish, antigenD10 was expressed by small groups of epithelial cells of the intestine of a shark, and generally by the epithelial and connective tissue cells of the gut and gills, and hepatocytes of one species of ray. The presence of antigenD10 in different tissues and species was confirmed by both an indirect ELISA and immunoblot analysis of tissue extracts. Our observations suggest that the D10 epitope characterises a subpopulation of mucus-secreting cells, predominantly of the foregut and associated organs, which has been conserved throughout terrestrial vertebrate evolution. Springer-Verlag, 2000 Antigen D10 Mucin Gastrointestinal Cell phenotype Phylogeny nationallicence Royce Simon G. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut Hughes Norman R. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut Binos Steve Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut Underwood John R. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut Bhathal Prithi S. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut https://doi.org/10.1007/s004180000171 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000171 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Royce Simon G. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut NATIONALLICENCE 700 1- Hughes Norman R. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut NATIONALLICENCE 700 1- Binos Steve Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut NATIONALLICENCE 700 1- Underwood John R. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut NATIONALLICENCE 700 1- Bhathal Prithi S. Department of Pathology, The University of Melbourne, Parkville, Victoria, Australia 3010 aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer