caa a22 4500 475774450 CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s004180000174 doi (NATIONALLICENCE)springer-10.1007/s004180000174 Lipids are a constitutive component of cytolytic granules [Elektronische Daten] [Vladimir Baranov, Olga Nagaeva, Sten Hammarström, Lucia Mincheva-Nilsson] Abstract.: Cytolytic granules are specific organelles of activated cytotoxic lymphocytes mediating storage and regulated excretion of lytic molecules for killing of target cells. A variety of the other granule components may also participate in granule-mediated cytotoxicity. In this study, the subcellular localization of lipids in the granules of human decidual CD56+ natural killer-like cells was determined by staining with malachite green aldehyde and imidazole-buffered osmium tetroxide. Lipids were shown, for the first time, to be a constitutive component of cytolytic granules. Lipids formed an additional structural microdomain, located between the granule-limiting membrane and the granule core. Images of the granules on serial sections suggested that intragranular lipids wrap the core. We speculate that granule lipids participate in packing of lytic molecules inside the granules, in autocrine signaling ending granule secretion, and in the killing process. Springer-Verlag, 2000 Lipids Cytotoxic granules Perforin/granzyme Fas ligand Cytotoxic cells NK cells γδTcells nationallicence Baranov Vladimir Department of Immunology, Umeå University, Sweden aut Nagaeva Olga Department of Clinical Immunology, Umeå University, 90185 Umeå, Sweden aut Hammarström Sten Department of Immunology, Umeå University, Sweden aut Mincheva-Nilsson Lucia Department of Clinical Immunology, Umeå University, 90185 Umeå, Sweden aut https://doi.org/10.1007/s004180000174 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000174 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Baranov Vladimir Department of Immunology, Umeå University, Sweden aut NATIONALLICENCE 700 1- Nagaeva Olga Department of Clinical Immunology, Umeå University, 90185 Umeå, Sweden aut NATIONALLICENCE 700 1- Hammarström Sten Department of Immunology, Umeå University, Sweden aut NATIONALLICENCE 700 1- Mincheva-Nilsson Lucia Department of Clinical Immunology, Umeå University, 90185 Umeå, Sweden aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer