caa a22 4500 475774485 CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s004180000179 doi (NATIONALLICENCE)springer-10.1007/s004180000179 König Karsten Laser Microscopy Division, Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben 7, 07743 Jena, Germany aut Laser tweezers and multiphoton microscopes in life sciences [Elektronische Daten] [Karsten König] Abstract.: Near infrared (NIR) laser microscopy enables optical micromanipulation, piconewton force determination, and sensitive fluorescence studies by laser tweezers. Otherwise, fluorescence images with high spatial and temporal resolution of living cells and tissues can be obtained via non-resonant fluorophore excitation with multiphoton NIR laser scanning microscopes. Furthermore, NIR femtosecond laser pulses at TW/cm2 intensities can be used to realize non-invasive contact-free surgery of nanometer-sized structures within living cells and tissues. Applications of these novel versatile NIR laser-based tools for the determination of motility forces, coenzyme and chlorophyll imaging, three-dimensional multigene detection, non-invasive optical sectioning of tissues ("optical biopsy"), functional protein imaging, and nanosurgery of chromosomes are described. Springer-Verlag, 2000 FISH FRET Laser tweezers Multiphoton microscopes Nanosurgery nationallicence https://doi.org/10.1007/s004180000179 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000179 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- König Karsten Laser Microscopy Division, Institute of Anatomy II, Friedrich Schiller University Jena, Teichgraben 7, 07743 Jena, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer