caa a22 4500 475774515 CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s004180050002 doi (NATIONALLICENCE)springer-10.1007/s004180050002 Microwaving for double indirect immunofluorescence with primary antibodies from the same species and for staining of mouse tissues with mouse monoclonal antibodies [Elektronische Daten] [D. Tornehave, D. M. Hougaard, L. -I. Larsson] Abstract : Many methods have been devised for double immunocytochemical staining. We now describe that moderate microwaving does not elute antibodies, but prevents their reactions with subsequently applied reagents. Thus, microwaving performed in between the first and second staining cycles permits double indirect immunofluorescence staining with antibodies raised in the same species. Moreover, microwaving also inhibits reactions with endogenous immunoglobulins present in extracellular compartments. This substantially reduces background in indirect immunostaining of mouse tissues with mouse monoclonal antibodies. Springer-Verlag Berlin Heidelberg, 2000 Tornehave D. Department for Pathobiology and Diagnostics, Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark, DK aut Hougaard D. M. Department of Clinical Biochemistry, Statens Serum Institut, Copenhagen, Denmark, DK aut Larsson L. -I Division of Cell Biology, Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Gronnegaardsvej 7, 1870 Frederiksberg C, Denmark e-mail: lail@kvl.dk Tel.: +45-35282851, Fax: +45-35282547, DK aut https://doi.org/10.1007/s004180050002 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050002 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tornehave D. Department for Pathobiology and Diagnostics, Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark, DK aut NATIONALLICENCE 700 1- Hougaard D. M. Department of Clinical Biochemistry, Statens Serum Institut, Copenhagen, Denmark, DK aut NATIONALLICENCE 700 1- Larsson L. -I Division of Cell Biology, Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Gronnegaardsvej 7, 1870 Frederiksberg C, Denmark e-mail: lail@kvl.dk Tel.: +45-35282851, Fax: +45-35282547, DK aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer