caa a22 4500 47577454X CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s004180050007 doi (NATIONALLICENCE)springer-10.1007/s004180050007 Peroxisomes in molluscs, characterization by subcellular fractionation combined with western blotting, immunohistochemistry, and immunocytochemistry [Elektronische Daten] [I. Cancio, A. Völkl, K. Beier, H. D. Fahimi, M. P. Cajaraville] Abstract : Peroxisomes of the digestive glands of mussels, Mytilus galloprovincialis Lmk, were investigated by immunoblotting and immunohistochemistry using rabbit antibodies against several mammalian hepatic peroxisomal proteins. Western blot analysis of main subcellular fractions revealed immunoreactive polypeptides with molecular weights comparable to those of the corresponding mammalian hepatic proteins. They could be localized to the peroxisomal matrix in the case of catalase, multifunctional enzyme (PH), and palmitoyl-CoA oxidase (AOX), and to the peroxisomal membrane in respect to PMP 70. The purification of peroxisomes by metrizamide density gradient centrifugation revealed the existence of two subpopulations with densities of 1.16 and 1.20 g cm-3 exhibiting different protein compositions. In paraffin sections, positive immunolabeling for catalase was distributed along the apical cytoplasm of the epithelia of digestive ducts and stomach and throughout the cytoplasm of digestive tubule cells. The peroxisomal β-oxidation enzymes, AOX and PH, also appeared predominantly in the ducts and the stomach epithelia with a weaker immunolabeling in the tubules. At the electron microscopic level a clear labeling with gold particles was observed in the peroxisomal matrix with the anti-guinea pig catalase antibody. In addition to peroxisomes, the anti-PH antibody also labeled the mitochondria. The similarity in the protein composition of molluscan and mammalian peroxisomes as revealed by the present study indicates that those proteins have been well conserved in evolution suggesting that functionally peroxisomes in molluscs could also be involved in the metabolism of lipids and in detoxification of xenobiotics. Thus, the antibodies tested could provide useful tools for detection of peroxisomal induction in molluscan biomonitoring programs for the assessment of aquatic environmental pollution. Springer-Verlag Berlin Heidelberg, 2000 Cancio I. Biologia Zelularra Atala, Zoologia eta Animali Zelulen Dinamika Saila, Euskal Herriko Unibertsitatea/Universidad del País Vasco, 644 PK, Bilbo 48080, Basque Country, Spain e-mail: zopcabem@lg.ehu.es Tel.: +34-94-6012697, Fax: +34-94-4648500, ES aut Völkl A. Institut für Anatomie und Zellbiologie II, Universität Heidelberg, 69120 Heidelberg, Germany, DE aut Beier K. Institut für Anatomie und Zellbiologie II, Universität Heidelberg, 69120 Heidelberg, Germany, DE aut Fahimi H. D. Institut für Anatomie und Zellbiologie II, Universität Heidelberg, 69120 Heidelberg, Germany, DE aut Cajaraville M. P. Biologia Zelularra Atala, Zoologia eta Animali Zelulen Dinamika Saila, Euskal Herriko Unibertsitatea/Universidad del País Vasco, 644 PK, Bilbo 48080, Basque Country, Spain e-mail: zopcabem@lg.ehu.es Tel.: +34-94-6012697, Fax: +34-94-4648500, ES aut https://doi.org/10.1007/s004180050007 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050007 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cancio I. Biologia Zelularra Atala, Zoologia eta Animali Zelulen Dinamika Saila, Euskal Herriko Unibertsitatea/Universidad del País Vasco, 644 PK, Bilbo 48080, Basque Country, Spain e-mail: zopcabem@lg.ehu.es Tel.: +34-94-6012697, Fax: +34-94-4648500, ES aut NATIONALLICENCE 700 1- Völkl A. Institut für Anatomie und Zellbiologie II, Universität Heidelberg, 69120 Heidelberg, Germany, DE aut NATIONALLICENCE 700 1- Beier K. Institut für Anatomie und Zellbiologie II, Universität Heidelberg, 69120 Heidelberg, Germany, DE aut NATIONALLICENCE 700 1- Fahimi H. D. Institut für Anatomie und Zellbiologie II, Universität Heidelberg, 69120 Heidelberg, Germany, DE aut NATIONALLICENCE 700 1- Cajaraville M. P. Biologia Zelularra Atala, Zoologia eta Animali Zelulen Dinamika Saila, Euskal Herriko Unibertsitatea/Universidad del País Vasco, 644 PK, Bilbo 48080, Basque Country, Spain e-mail: zopcabem@lg.ehu.es Tel.: +34-94-6012697, Fax: +34-94-4648500, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer