caa a22 4500 475774558 CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s004180050005 doi (NATIONALLICENCE)springer-10.1007/s004180050005 Differential expression of dihydropyrimidinase-related protein genes in developing and adult enteric nervous system [Elektronische Daten] [H. Inagaki, Y. Kato, N. Hamajima, M. Nonaka, M. Sasaki, T. Eimoto] Abstract : Dihydropyrimidinase-related proteins (DRPs) are involved in axonal outgrowth and pathfinding. However, little is known about their significance in the enteric nervous system (ENS), the largest and most complex division of the peripheral nervous system. Using in situ hybridization (ISH) and northern blotting, we examined mRNA expression of DRP-1-4 transcripts in the developing and adult mouse digestive tract and in the adult human colon. ISH detected the mouse DRP-3 transcript in the developing ENS on embryonic day (E)12 and at the later stages as well as in the adult intestine. Mouse DRP-1 and -2 transcripts appeared at E14. DRP-2 transcript was also detected in the adult intestine although DRP-1 expression was lower in the adult. DRP-4 gene was not expressed in the ENS during development or adulthood whereas the signal was apparent in the developing and adult central nervous system (CNS). The DRP expression pattern in the human colon was similar to that of the mouse large intestine. Northern blot analysis showed that DRPs were differentially expressed in the mouse and human intestines, supporting the results of ISH. These data suggest that DRPs play a role not only in the CNS but also in the ENS. Springer-Verlag Berlin Heidelberg, 2000 Inagaki H. Department of Pathology, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan e-mail: hinagaki@med.nagoya-cu.ac.jp Tel.: +81-52-8538161, Fax: +81-52-8514166, JP aut Kato Y. Department of Biochemistry, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan, JP aut Hamajima N. Department of Pediatrics, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan, JP aut Nonaka M. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan, JP aut Sasaki M. Department of Biochemistry, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan, JP aut Eimoto T. Department of Pathology, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan e-mail: hinagaki@med.nagoya-cu.ac.jp Tel.: +81-52-8538161, Fax: +81-52-8514166, JP aut https://doi.org/10.1007/s004180050005 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050005 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inagaki H. Department of Pathology, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan e-mail: hinagaki@med.nagoya-cu.ac.jp Tel.: +81-52-8538161, Fax: +81-52-8514166, JP aut NATIONALLICENCE 700 1- Kato Y. Department of Biochemistry, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan, JP aut NATIONALLICENCE 700 1- Hamajima N. Department of Pediatrics, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan, JP aut NATIONALLICENCE 700 1- Nonaka M. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan, JP aut NATIONALLICENCE 700 1- Sasaki M. Department of Biochemistry, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan, JP aut NATIONALLICENCE 700 1- Eimoto T. Department of Pathology, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan e-mail: hinagaki@med.nagoya-cu.ac.jp Tel.: +81-52-8538161, Fax: +81-52-8514166, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer