caa a22 4500 475774582 CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004180000155 doi (NATIONALLICENCE)springer-10.1007/s004180000155 Cytoskeletal involvement during hypo-osmotic swelling and volume regulation in cultured chick cardiac myocytes [Elektronische Daten] [Terje H. Larsen, Helge Dalen, Robert Boyle, Marta M. Souza, Melvyn Lieberman] Abstract.: The membrane skeleton in spherical cardiac myocytes subjected to hypo-osmotic challenge was examined by laser scanning confocal microscopy. A distinct cortical layer intimately localized under the plasmalemma was revealed for spectrin and actin (including filamentous actin and α-sarcomeric actin). Desmin filaments were abundant and in close contact with the plasmalemma. During swelling and subsequent regulatory volume decrease (RVD) the structural integrity of these cytoskeletal elements remained intact, and the close association between actin and plasmalemma persisted as confirmed by double immunolabeling. Subplasmalemmal β-tubulin labeling was sparse. Hypo-osmotic conditions disrupted the microtubules and depolymerized tubulin. Neither pretreatment with taxol nor with colchicine, resulted in any effect on cell volume regulation. The present results show that actin, desmin, and spectrin contribute to a subplasmalemmal cytoskeletal network in spherical cardiac myocytes, and that this membrane skeleton remains structurally intact during swelling and RVD. It is suggested that the integrity of this membrane skeleton is important for stabilization of the plasmalemma and the membrane-integrated proteins during hypo-osmotic challenge, and that it may participate in the regulation of the cell volume. Springer-Verlag, 2000 Regulatory volume decrease Antibodies Immunofluorescence Laser scanning confocal microscopy Membrane skeleton nationallicence Larsen Terje H. Experimental Cardiology Unit, Department of Radiology, The Gade Institute, University of Bergen, 5021 Bergen, Norway aut Dalen Helge Department of Cell Biology, Division of Physiology, Duke University Medical Center, Durham, NC 27710, USA aut Boyle Robert Department of Cell Biology, Division of Physiology, Duke University Medical Center, Durham, NC 27710, USA aut Souza Marta M. Department of Cell Biology, Division of Physiology, Duke University Medical Center, Durham, NC 27710, USA aut Lieberman Melvyn Department of Cell Biology, Division of Physiology, Duke University Medical Center, Durham, NC 27710, USA aut https://doi.org/10.1007/s004180000155 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000155 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Larsen Terje H. Experimental Cardiology Unit, Department of Radiology, The Gade Institute, University of Bergen, 5021 Bergen, Norway aut NATIONALLICENCE 700 1- Dalen Helge Department of Cell Biology, Division of Physiology, Duke University Medical Center, Durham, NC 27710, USA aut NATIONALLICENCE 700 1- Boyle Robert Department of Cell Biology, Division of Physiology, Duke University Medical Center, Durham, NC 27710, USA aut NATIONALLICENCE 700 1- Souza Marta M. Department of Cell Biology, Division of Physiology, Duke University Medical Center, Durham, NC 27710, USA aut NATIONALLICENCE 700 1- Lieberman Melvyn Department of Cell Biology, Division of Physiology, Duke University Medical Center, Durham, NC 27710, USA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer