caa a22 4500 475774612 CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004180000136 doi (NATIONALLICENCE)springer-10.1007/s004180000136 The metencephalic floor plate of chick embryos expresses two secretory glycoproteins homologous with the two glycoproteins secreted by the subcommissural organ [Elektronische Daten] [M. Angeles del Brío, Pedro Riera, Rosa I. Muñoz, Hernán Montecinos, Esteban M. Rodríguez] Abstract.: The nature and the function of the compounds secreted by the floor plate (FP) of the metencephalon are little known. The FP cells of the hindbrain react with antibodies (AFRU) against the glycoproteins secreted by the subcommissural organ (SCO). One of the these proteins, RF-Gly I, is a 540-kDa core glycosylated protein. The aims of the present investigation were to identify by immunoblot the AFRU-immunoreactive compound secreted by the FP of chick embryos, to establish temporal and regional patterns of this secretory activity, and to obtain information about the fate of these compounds. It was established that the SCO and FP of chick embryos secrete two AFRU-immunoreactive compounds of 540 and 230 kDa. The two compounds secreted by the FP have been designated as FP-Gly I and FP-Gly II. The expression of these proteins was circumscribed to the metencephalic FP, and occurred from HH 29 to HH 36. Within the FP cells, FP-Gly I and FP-Gly II were confined to the supranuclear and apical regions, which under the electron microscope displayed numerous cisternae of the rough endoplasmic reticulum and granules. Aggregates of AFRU-immunoreactive material appeared on the free surface of the FP. The possibility that FP-Gly I and FP-Gly II are released into the ventricle to reach distant targets is discussed. Springer-Verlag, 2000 del Brío M. Angeles Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, Spain aut Riera Pedro Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, Spain aut Muñoz Rosa I. Instituto de Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile aut Montecinos Hernán Instituto de Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile aut Rodríguez Esteban M. Instituto de Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile aut https://doi.org/10.1007/s004180000136 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000136 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- del Brío M. Angeles Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, Spain aut NATIONALLICENCE 700 1- Riera Pedro Departamento de Morfología y Biología Celular, Facultad de Medicina, Universidad de Oviedo, Spain aut NATIONALLICENCE 700 1- Muñoz Rosa I. Instituto de Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile aut NATIONALLICENCE 700 1- Montecinos Hernán Instituto de Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile aut NATIONALLICENCE 700 1- Rodríguez Esteban M. Instituto de Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer