caa a22 4500 475774655 CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s004180000146 doi (NATIONALLICENCE)springer-10.1007/s004180000146 Spermatogenesis proceeds normally in mice without linker histone H1t [Elektronische Daten] [Birgit Drabent, Paul Saftig, Christa Bode, Detlef Doenecke] Abstract.: The histone gene H1t is expressed exclusively in pachytene spermatocytes of the testis. In this report we have eliminated the single copy H1t gene by homologous recombination from the mouse genome to analyse the function of the H1t protein during spermatogenesis. Mice homozygous for the mutated H1t gene locus developed normally and showed no anatomic abnormalities until the adult stage. In addition, H1t-deficient mice were fertile and reproduced as wild-type mice. The process of spermatogenesis and the testicular morphology remained unchanged in the absence of H1t. RNase protection analysis demonstrated that H1.1, H1.2 and H1.4 histone gene expression is enhanced during spermatogenesis in H1t-deficient mice. Springer-Verlag, 2000 Chromatin structure Linker histones H1t-deficient mice Spermatogenesis nationallicence Drabent Birgit Zentrum Biochemie und Molekulare Zellbiologie, Abt. Molekularbiologie, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany aut Saftig Paul Zentrum Biochemie und Molekulare Zellbiologie, Abt. Biochemie II, Universität Göttingen, Heinrich-Düker-Weg 12, 37073 Göttingen, Germany aut Bode Christa Zentrum Biochemie und Molekulare Zellbiologie, Abt. Molekularbiologie, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany aut Doenecke Detlef Zentrum Biochemie und Molekulare Zellbiologie, Abt. Molekularbiologie, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany aut https://doi.org/10.1007/s004180000146 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000146 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Drabent Birgit Zentrum Biochemie und Molekulare Zellbiologie, Abt. Molekularbiologie, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany aut NATIONALLICENCE 700 1- Saftig Paul Zentrum Biochemie und Molekulare Zellbiologie, Abt. Biochemie II, Universität Göttingen, Heinrich-Düker-Weg 12, 37073 Göttingen, Germany aut NATIONALLICENCE 700 1- Bode Christa Zentrum Biochemie und Molekulare Zellbiologie, Abt. Molekularbiologie, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany aut NATIONALLICENCE 700 1- Doenecke Detlef Zentrum Biochemie und Molekulare Zellbiologie, Abt. Molekularbiologie, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer