caa a22 4500 47577468X CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s004180050441 doi (NATIONALLICENCE)springer-10.1007/s004180050441 Characterization of caveolins from human knee joint cartilage: expression of caveolin-1, -2, and -3 in chondrocytes and association with integrin β1 [Elektronische Daten] [W. Schwab, M. Kasper, J. M. Gavlik, E. Schulze, R. H. W. Funk, M. Shakibaei] Abstract : Interactions between the extracellular matrix (ECM) and chondrocytes are of great importance for structure and function of cartilage. The present study was undertaken to answer the question whether caveolins take part in integrin-mediated cell-ECM interactions in the human cartilage. In samples of human knee joint cartilage, we detected the caveolin subtypes -1, -2, and -3 by immunohistochemical methods. Double-label experiments revealed a colocalization of caveolin with β1-integrin. Results of immunoprecipitation and immunoblotting assays show that β1-integrins associate with all three caveolin subtypes in human chondrocytes and indicate that they are part of the same complexes. Furthermore, immunoelectron microscopy shows the localization of β1-integrin in caveolae-like structures of the cell membrane. The data stimulate further investigations on the role of the caveolin-integrin complex for integrin-mediated signaling pathways in chondrocytes. Springer-Verlag Berlin Heidelberg, 2000 Schwab W. Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany e-mail: Wolfgang.Schwab@mailbox.tu-dresden.de Tel.: +49-351-4583525, Fax: +49-351-4585329, DE aut Kasper M. Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany e-mail: Wolfgang.Schwab@mailbox.tu-dresden.de Tel.: +49-351-4583525, Fax: +49-351-4585329, DE aut Gavlik J. M. Clinic of Surgery, Division of Reconstructive Surgery, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany, DE aut Schulze E. Clinic of Surgery, Division of Reconstructive Surgery, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany, DE aut Funk R. H. W. Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany e-mail: Wolfgang.Schwab@mailbox.tu-dresden.de Tel.: +49-351-4583525, Fax: +49-351-4585329, DE aut Shakibaei M. Institute of Anatomy, Free University of Berlin, Königin-Louise-Strasse 15, 14195 Berlin, Germany, DE aut https://doi.org/10.1007/s004180050441 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050441 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schwab W. Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany e-mail: Wolfgang.Schwab@mailbox.tu-dresden.de Tel.: +49-351-4583525, Fax: +49-351-4585329, DE aut NATIONALLICENCE 700 1- Kasper M. Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany e-mail: Wolfgang.Schwab@mailbox.tu-dresden.de Tel.: +49-351-4583525, Fax: +49-351-4585329, DE aut NATIONALLICENCE 700 1- Gavlik J. M. Clinic of Surgery, Division of Reconstructive Surgery, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany, DE aut NATIONALLICENCE 700 1- Schulze E. Clinic of Surgery, Division of Reconstructive Surgery, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany, DE aut NATIONALLICENCE 700 1- Funk R. H. W. Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany e-mail: Wolfgang.Schwab@mailbox.tu-dresden.de Tel.: +49-351-4583525, Fax: +49-351-4585329, DE aut NATIONALLICENCE 700 1- Shakibaei M. Institute of Anatomy, Free University of Berlin, Königin-Louise-Strasse 15, 14195 Berlin, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer