caa a22 4500 475774698 CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s004180050436 doi (NATIONALLICENCE)springer-10.1007/s004180050436 Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization [Elektronische Daten] [R.P.M. van Gijlswijk, M.P.C. van de Corput, V. Bezrookove, J. Wiegant, H. J. Tanke, A. K. Raap] Abstract : A method is presented to conjugate horseradish peroxidase (HRP) to oligodeoxynucleotides for fluorescence in situ hybridization assays employing tyramide signal amplification (TSA). HRP is covalently bound to the oligonucleotide by thiol ether linkage and purified by high-performance liquid chromatography. With TSA detection, a single HRP-labeled oligonucleotide probe is sufficient for in situ detection of clustered DNA repeat sequences with a degree of repetition between 20 and 50. Springer-Verlag Berlin Heidelberg, 2000 van Gijlswijk R.P.M. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut van de Corput M.P.C. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut Bezrookove V. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut Wiegant J. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut Tanke H. J. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut Raap A. K. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut https://doi.org/10.1007/s004180050436 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050436 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Gijlswijk R.P.M. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut NATIONALLICENCE 700 1- van de Corput M.P.C. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut NATIONALLICENCE 700 1- Bezrookove V. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut NATIONALLICENCE 700 1- Wiegant J. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut NATIONALLICENCE 700 1- Tanke H. J. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut NATIONALLICENCE 700 1- Raap A. K. Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands Tel.: +31-71-5276187, Fax: +31-71-5276180, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer