caa a22 4500 47577471X CHVBK 20180406123627.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s004180050438 doi (NATIONALLICENCE)springer-10.1007/s004180050438 Expression of the apoptosis-inducing ligands FasL and TRAIL in malignant and benign human breast tumors [Elektronische Daten] [C. Herrnring, T. Reimer, U. Jeschke, J. Makovitzky, K. Krüger, B. Gerber, D. Kabelitz, K. Friese] Abstract : Apoptosis-inducing ligands such as Fas ligand (FasL) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) have been found to play an important role in cell regulation. Different malignant tumors show an altered expression of these ligands and their respective receptors compared to normal tissues. The purpose of this study was therefore to investigate expression of TRAIL, FasL, and its receptor Fas on protein and mRNA levels in breast carcinomas (n=40), fibroadenomas (n=7), and normal breast tissues (n=5). Immunohistochemical reaction demonstrated that FasL was strongly expressed in breast cancer tissues (34/40) while only one fibroadenoma and one normal breast tissue reacted weakly positive for FasL. All fibroadenomas and normal breast tissues as well as the majority of breast cancer tissues expressed Fas on protein level. Quantitative RT-PCR analysis detected high expression of FasL mRNA in breast cancer tissues and fibroadenomas, whereas fibroadenomas showed the highest Fas mRNA copy numbers, followed by breast cancer tissues and normal breast tissues (P<0.05). Compared to FasL expression, TRAIL could be detected in less breast cancer tissues on protein level (21/40) and was found in only one fibroadenoma and none of the normal breast tissues. Thus, it can be concluded that malignant breast tumors show an altered expression of the two apoptosis-inducing ligands FasL and TRAIL. Springer-Verlag Berlin Heidelberg, 2000 Herrnring C. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut Reimer T. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut Jeschke U. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut Makovitzky J. Department of Pathology, University of Rostock, Germany, DE aut Krüger K. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut Gerber B. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut Kabelitz D. Department of Immunology, University of Kiel, Germany, DE aut Friese K. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut https://doi.org/10.1007/s004180050438 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050438 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Herrnring C. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut NATIONALLICENCE 700 1- Reimer T. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut NATIONALLICENCE 700 1- Jeschke U. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut NATIONALLICENCE 700 1- Makovitzky J. Department of Pathology, University of Rostock, Germany, DE aut NATIONALLICENCE 700 1- Krüger K. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut NATIONALLICENCE 700 1- Gerber B. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut NATIONALLICENCE 700 1- Kabelitz D. Department of Immunology, University of Kiel, Germany, DE aut NATIONALLICENCE 700 1- Friese K. Department of Obstetrics and Gynecology, University of Rostock, Doberaner Strasse 142, 18055 Rostock, Germany e-mail: klaus.friese@med.uni-rostock.de Tel.: +49-381-4948101, Fax: +49-381-4948102, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer