caa a22 4500 475774744 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s004180050442 doi (NATIONALLICENCE)springer-10.1007/s004180050442 Ultrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrix [Elektronische Daten] [R. G. Aktas, R. J. Kayton] Abstract : Basic fibroblast growth factor (bFGF) is thought to play an important role in normal tissue repair and wound healing. It is a potent mitogenic and chemotactic factor for fibroblasts, regulating proliferation and extracellular matrix (ECM) production by these cells. In this study, we present morphologic evidence of the ultrastructural location of bFGF in fibroblasts and ECM using several antibodies, tissues, and species. Distinct labeling is seen in the nuclei of fibroblasts and some labeling in the cytosol. Immunolabeling of the cytosol excludes organelles involved in the usual secretory pathway, such as rough endoplasmic reticulum, Golgi apparatus, and secretory vacuoles. The same labeling is observed with either polyclonal or monoclonal antibodies. We suggest that bFGF functions as a nuclear protein in fibroblasts and is not secreted by a normal secretory pathway. Fibroblasts may export bFGF via unique cellular pathways that are clearly distinct from classic signal peptide mediated secretion. This may provide a source for ECM-resident bFGF. The same antibodies show different labeling intensity in the ECM. This protein, through integration into the ECM, may act as a local regulator and promote regeneration of these tissues after wounding. Direct evidence is the dramatic reduction of bFGF labeling in axotomized rat ECM collagen fibers versus control animals. Springer-Verlag Berlin Heidelberg, 2000 Aktas R. G. Trakya University, Faculty of Medicine, Pathology Department, 22030 Edirne, Turkey, TR aut Kayton R. J. Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, C.R.O.E.T., L606, Portland, OR 97201, USA e-mail: kayton@ohsu.edu Tel.: +1-503-4942504, Fax: +1-503-4946831, US aut https://doi.org/10.1007/s004180050442 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050442 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aktas R. G. Trakya University, Faculty of Medicine, Pathology Department, 22030 Edirne, Turkey, TR aut NATIONALLICENCE 700 1- Kayton R. J. Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, C.R.O.E.T., L606, Portland, OR 97201, USA e-mail: kayton@ohsu.edu Tel.: +1-503-4942504, Fax: +1-503-4946831, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer