caa a22 4500 475774787 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004180000135 doi (NATIONALLICENCE)springer-10.1007/s004180000135 Immunological detection of polycystin-1 in human kidney [Elektronische Daten] [Jeroen Nauta, Miriam A. Goedbloed, Ans M.W. van den Ouweland, Mark Nellist, André T. Hoogeveen] Polycystin-1 is the protein product of the PKD1 gene. Mutations in this gene are responsible for most cases of polycystic kidney disease, but little is known about how these mutations lead to the development of cysts. Indeed, even less is known about the normal role of polycystin-1 in the kidney. The cellular localization of polycystin-1 has been the subject of intense investigation by many groups, including ours. In this report we describe our results and compare our data with those of others. We generated 14 different polyclonal antisera against fragments of the predicted 462-kDa polycystin-1 molecule to enable us to investigate the expression of polycystin-1 in cells and tissues by immunocytochemistry, western blotting, and immunoprecipitation. Our antibodies readily recognized a 134-kDa polycystin-1 fragment overexpressed in COS cells and stained the epithelial cells of fetal, adult, and cystic kidney sections with the same pattern as reported by others. However, further investigations revealed that this pattern was not specific for polycystin-1. We could not unequivocally detect polycystin-1 in vivo, either by immunoblotting or immunocytochemistry. Therefore our studies do not support the reported pattern of polycystin-1 expression in the kidney. Springer-Verlag, 2000 Nauta Jeroen Department of Pediatric Nephrology, Sophia Children's Hospital, Molewaterplein 50, 3015 GJ Rotterdam, The Netherlands aut Goedbloed Miriam A. Department of Pediatric Nephrology, Sophia Children's Hospital, Molewaterplein 50, 3015 GJ Rotterdam, The Netherlands aut van den Ouweland Ans M.W. Department of Clinical Genetics, Erasmus Medical Center Rotterdam, Molewaterplein 60, Rotterdam, The Netherlands aut Nellist Mark Department of Clinical Genetics, Erasmus Medical Center Rotterdam, Molewaterplein 60, Rotterdam, The Netherlands aut Hoogeveen André T. Department of Clinical Genetics, Erasmus Medical Center Rotterdam, Molewaterplein 60, Rotterdam, The Netherlands aut Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 303-311 0948-6143 113:4<303 2000 113 418 https://doi.org/10.1007/s004180000135 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000135 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nauta Jeroen Department of Pediatric Nephrology, Sophia Children's Hospital, Molewaterplein 50, 3015 GJ Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Goedbloed Miriam A. Department of Pediatric Nephrology, Sophia Children's Hospital, Molewaterplein 50, 3015 GJ Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- van den Ouweland Ans M.W. Department of Clinical Genetics, Erasmus Medical Center Rotterdam, Molewaterplein 60, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Nellist Mark Department of Clinical Genetics, Erasmus Medical Center Rotterdam, Molewaterplein 60, Rotterdam, The Netherlands aut NATIONALLICENCE 700 1- Hoogeveen André T. Department of Clinical Genetics, Erasmus Medical Center Rotterdam, Molewaterplein 60, Rotterdam, The Netherlands aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 303-311 0948-6143 113:4<303 2000 113 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer