caa a22 4500 475774795 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004180000128 doi (NATIONALLICENCE)springer-10.1007/s004180000128 A preformed basal lamina alters the metabolism and distribution of hyaluronan in epidermal keratinocyte "organotypic" cultures grown on collagen matrices [Elektronische Daten] [Raija H. Tammi, Markku I. Tammi, Vincent C. Hascall, Michael Hogg, Sanna Pasonen, Donald K. MacCallum] A rat epidermal keratinocyte (REK) line which exhibits histodifferentiation nearly identical to the native epidermis when cultured at an air-liquid interface was used to study the metabolism of hyaluronan, the major intercellular macromolecule present in basal and spinous cell layers. Two different support matrices were used: reconstituted collagen fibrils with and without a covering basal lamina previously deposited by canine kidney cells. REKs formed a stratified squamous, keratinized epithelium on both support matrices. Hyaluronan and its receptor, CD44, colocalized in the basal and spinous layers similar to their distribution in the native epidermis. Most (approximately 75%) of the hyaluronan was retained in the epithelium when a basal lamina was present while most (approximately 80%) diffused out of the epithelium in its absence. While REKs on the two matrices synthesized hyaluronan at essentially the same rate, catabolism of this macromolecule was much higher in the epithelium on the basal lamina (half-life approximately 1day, similar to its half-life in native human epidermis). The formation of a true epidermal compartment in culture bounded by the cornified layer on the surface and the basal lamina subjacent to the basal cells provides a good model within which to study epidermal metabolism. Springer-Verlag, 2000 Tammi Raija H. Department of Anatomy, University of Kuopio, 70211 Kuopio, Finland aut Tammi Markku I. Department of Anatomy, University of Kuopio, 70211 Kuopio, Finland aut Hascall Vincent C. Department of Biomedical Engineering, ND 20, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA aut Hogg Michael Department of Biomedical Engineering, ND 20, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA aut Pasonen Sanna Department of Anatomy, University of Kuopio, 70211 Kuopio, Finland aut MacCallum Donald K. Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan 48109-0616, USA aut Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 265-277 0948-6143 113:4<265 2000 113 418 https://doi.org/10.1007/s004180000128 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000128 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tammi Raija H. Department of Anatomy, University of Kuopio, 70211 Kuopio, Finland aut NATIONALLICENCE 700 1- Tammi Markku I. Department of Anatomy, University of Kuopio, 70211 Kuopio, Finland aut NATIONALLICENCE 700 1- Hascall Vincent C. Department of Biomedical Engineering, ND 20, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA aut NATIONALLICENCE 700 1- Hogg Michael Department of Biomedical Engineering, ND 20, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA aut NATIONALLICENCE 700 1- Pasonen Sanna Department of Anatomy, University of Kuopio, 70211 Kuopio, Finland aut NATIONALLICENCE 700 1- MacCallum Donald K. Department of Anatomy and Cell Biology, University of Michigan, Ann Arbor, Michigan 48109-0616, USA aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 265-277 0948-6143 113:4<265 2000 113 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer