caa a22 4500 475774809 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004180000134 doi (NATIONALLICENCE)springer-10.1007/s004180000134 Enzyme histochemistry on human placental trophoblasts: the effect of fixation delay on enzyme activity [Elektronische Daten] [Shigeki Matsubara, Toshihiro Takizawa, Ikuo Sato] We examined the effect of time delay in tissue fixation on enzyme histochemically detectable enzyme activity and its localization in term human placental trophoblasts. Four placental enzymes, alkaline phosphatase, acid phosphatase, glucose-6-phosphatase, and cytochromec oxidase, were studied. A fixation delay of 15min did not markedly alter the activity or distribution pattern of the four enzymes, excepted for a slight reduction in cytochromec oxidase activity and the appearance of dilated endoplasmic reticula positive for glucose-6-phosphatase. A fixation delay of 60min abolished cytochromec oxidase activity, but the activities of the other three enzymes remained positive. When the placental tissue was stored at 4°C without cutting for 24h before fixation, cell degeneration occurred. However, alkaline phosphatase activity was still clearly demonstrable. In enzyme histochemistry, "immediate" fixation is superior, but even if this cannot be performed, the placentas, especially when they are from patients with rare disorders, should not be discarded. Observations made here will be useful for clinician's attempting enzyme histochemistry in organs other than the placenta. Springer-Verlag, 2000 Matsubara Shigeki Department of Obstetrics and Gynecology, Jichi Medical School, Minamikawachi-machi 329-04, Kawachi-gun, Tochigi 329-0498, Japan aut Takizawa Toshihiro Department of Anatomy, Jichi Medical School, Tochigi, Japan aut Sato Ikuo Department of Obstetrics and Gynecology, Jichi Medical School, Minamikawachi-machi 329-04, Kawachi-gun, Tochigi 329-0498, Japan aut Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 287-292 0948-6143 113:4<287 2000 113 418 https://doi.org/10.1007/s004180000134 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000134 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsubara Shigeki Department of Obstetrics and Gynecology, Jichi Medical School, Minamikawachi-machi 329-04, Kawachi-gun, Tochigi 329-0498, Japan aut NATIONALLICENCE 700 1- Takizawa Toshihiro Department of Anatomy, Jichi Medical School, Tochigi, Japan aut NATIONALLICENCE 700 1- Sato Ikuo Department of Obstetrics and Gynecology, Jichi Medical School, Minamikawachi-machi 329-04, Kawachi-gun, Tochigi 329-0498, Japan aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 287-292 0948-6143 113:4<287 2000 113 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer