caa a22 4500 475774833 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004180000140 doi (NATIONALLICENCE)springer-10.1007/s004180000140 Tissue transglutaminase in the small intestine of the mouse as a marker for apoptotic cells. Colocalization with DNA fragmentation [Elektronische Daten] [A.P. Aschoff, E. Günther, G.F. Jirikowski] Besides the morphological changes in cells undergoing apoptosis, such as chromatin condensation and cell shrinkage, histological demonstration of DNA fragmentation by in situ end labeling (ISEL) has been widely used for the demonstration of apoptotic cells in tissue sections. Although DNA fragmentation can be demonstrated in apoptotic cells and apoptotic bodies in most cases, there is no clear correlation of ISEL staining with apoptosis. It has often been demonstrated that, in many morphologically intact cells, nuclei with fragmented DNA can be found. Thus staining with ISEL for the detection of apoptosis is useful only in connection with other markers for apoptosis as, for example, characteristic morphological changes. Here we show that tissue transglutaminase protein is unequivocally expressed in apoptotic enterocytes as shown by DNA fragmentation and morphology. Tissue transglutaminase is not expressed in enterocytes with healthy morphology, although DNA fragmentation can be demonstrated in these cells. Thus the immunohistochemical demonstration of tissue transglutaminase may serve as a simple marker for apoptotic epithelial cells in tissue sections. Springer-Verlag, 2000 Cell death Enterocytes Proliferation Differentiation ISEL nationallicence Aschoff A.P. Department of Anatomy II, FSU-Jena, Teichgraben 7, 07743 Jena, Germany aut Günther E. Department of Anatomy II, FSU-Jena, Teichgraben 7, 07743 Jena, Germany aut Jirikowski G.F. Department of Anatomy II, FSU-Jena, Teichgraben 7, 07743 Jena, Germany aut Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 313-317 0948-6143 113:4<313 2000 113 418 https://doi.org/10.1007/s004180000140 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000140 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aschoff A.P. Department of Anatomy II, FSU-Jena, Teichgraben 7, 07743 Jena, Germany aut NATIONALLICENCE 700 1- Günther E. Department of Anatomy II, FSU-Jena, Teichgraben 7, 07743 Jena, Germany aut NATIONALLICENCE 700 1- Jirikowski G.F. Department of Anatomy II, FSU-Jena, Teichgraben 7, 07743 Jena, Germany aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 313-317 0948-6143 113:4<313 2000 113 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer