caa a22 4500 475774841 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004180000137 doi (NATIONALLICENCE)springer-10.1007/s004180000137 In situ detection of apoptosis in dental and periodontal tissues of the adult mouse using annexin-V-biotin [Elektronische Daten] [A.L.J.J. Bronckers, S.W. Goei, E. Dumont, D.M. Lyaruu, J.H.M. Wöltgens, W.L. van Heerde, C.P.M. Reutelingsperger, S.M. van den Eijnde] An early event in apoptosis is exposure of phosphatidylserine, an aminophospholipid normally present in the inner leaflet of the plasma membranes, at the outer leaflet of the plasma membrane facing the extracellular space. AnnexinV (Anx-V) is a 35-kDa protein with high affinity for phosphatidylserine, which can be applied to detect apoptosis. We injected biotin-labelled Anx-V intravenously in adult mice and examined the tissue distribution of Anx-V-labelled cells in dental and periodontal tissues using ABC-peroxidase histochemistry. In the continuously erupting incisors, strong and frequent immunostaining was observed in transitional stage and late maturation stage ameloblasts with less frequent staining in preameloblasts. Frequency of staining in odontoblasts and pulp cells was low but increased slightly at older stages of dentinogenesis. Labelling was also seen in phagocytic or phagocytic-like cells in the enamel organ and pulp. A positive staining was furthermore found in fibroblasts of the periodontal ligament in continuously erupting incisors and in fully erupted molar teeth. Staining intensity and the number of positive cells were enhanced by antigen retrieval using high-pressure cooking. We conclude that Anx-V-biotin labels dental cells in early stages of cell death and indirectly cells that have ingested labelled apoptotic cells during the course of the experiment. The data confirm that during amelogenesis most cell death occurs in transitional stage and late maturation stage ameloblasts. Thus, labelling with Anx-V is a useful marker for studying cell death and the dynamics of clearance of apoptotic cells during tooth development. Springer-Verlag, 2000 Bronckers A.L.J.J. Department of Oral Cell Biology, ACTA, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands aut Goei S.W. Department of Oral Cell Biology, ACTA, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands aut Dumont E. Department of Cardiology, University of Maastricht, Universiteitsingel 50, 6200 MD Maastricht, The Netherlands aut Lyaruu D.M. Department of Oral Cell Biology, ACTA, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands aut Wöltgens J.H.M. Department of Oral Cell Biology, ACTA, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands aut van Heerde W.L. Department of Biochemistry, Cardiovascular Research Institute University of Maastricht, Universiteitsingel 50, Maastricht, The Netherlands aut Reutelingsperger C.P.M. Department of Biochemistry, Cardiovascular Research Institute University of Maastricht, Universiteitsingel 50, Maastricht, The Netherlands aut van den Eijnde S.M. MGG Department of Clinical Genetics, Erasmus University Medical School, 3000 DR Rotterdam, The Netherlands aut Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 293-301 0948-6143 113:4<293 2000 113 418 https://doi.org/10.1007/s004180000137 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000137 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bronckers A.L.J.J. Department of Oral Cell Biology, ACTA, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Goei S.W. Department of Oral Cell Biology, ACTA, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Dumont E. Department of Cardiology, University of Maastricht, Universiteitsingel 50, 6200 MD Maastricht, The Netherlands aut NATIONALLICENCE 700 1- Lyaruu D.M. Department of Oral Cell Biology, ACTA, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Wöltgens J.H.M. Department of Oral Cell Biology, ACTA, Vrije Universiteit, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- van Heerde W.L. Department of Biochemistry, Cardiovascular Research Institute University of Maastricht, Universiteitsingel 50, Maastricht, The Netherlands aut NATIONALLICENCE 700 1- Reutelingsperger C.P.M. Department of Biochemistry, Cardiovascular Research Institute University of Maastricht, Universiteitsingel 50, Maastricht, The Netherlands aut NATIONALLICENCE 700 1- van den Eijnde S.M. MGG Department of Clinical Genetics, Erasmus University Medical School, 3000 DR Rotterdam, The Netherlands aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 293-301 0948-6143 113:4<293 2000 113 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer