caa a22 4500 47577485X CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004180000131 doi (NATIONALLICENCE)springer-10.1007/s004180000131 The congenital chloride diarrhea gene is expressed in seminal vesicle, sweat gland, inflammatory colon epithelium, and in some dysplastic colon cells [Elektronische Daten] [Siru Haila, Ulpu Saarialho-Kere, Marja-Liisa Karjalainen-Lindsberg, Hannes Lohi, Kristiina Airola, Christer Holmberg, Johanna Hästbacka, Juha Kere, Pia Höglund] Congenital chloride diarrhea (CLD) is an autosomal recessive disorder of intestinal electrolyte transportation caused by mutations in the anion transporter protein encoded by the down-regulated in adenoma (DRA), or CLD, gene. In this study, in situ hybridization and immunohistochemistry were performed to investigate the expression of CLD in extraintestinal normal epithelia and in intestinal inflammatory and neoplastic epithelia. The expression of the closely related anion transporter diastrophic dysplasia sulfate transporter, DTDST, was also examined and compared with that of CLD in colon. The only extraintestinal tissues showing CLD expression were eccrine sweat glands and seminal vesicles. In inflammatory bowel disease and ischemic colitis, expression of CLD mRNA in colon epithelium was similar to histologically normal colon epithelium, but the protein was found deeper in crypts, including proliferative epithelial cells. In intestinal tumors, the expression pattern of CLD was dependent on the differentiation status of the tissue studied: epithelial polyps with no or minor dysplasia showed abundant expression, whereas adenocarcinomas were negative. The DTDST gene was abundantly expressed in the upper crypt epithelium of colonic mucosa. Springer-Verlag, 2000 Haila Siru Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut Saarialho-Kere Ulpu Department of Dermatology, 00029 Helsinki University Central Hospital, Finland aut Karjalainen-Lindsberg Marja-Liisa Department of Pathology, Haartman Institute, 00014 University of Helsinki, Finland aut Lohi Hannes Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut Airola Kristiina Department of Dermatology, 00029 Helsinki University Central Hospital, Finland aut Holmberg Christer Hospital for Children and Adolescents, 00029 Helsinki University Central Hospital, Finland aut Hästbacka Johanna Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut Kere Juha Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut Höglund Pia Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 279-286 0948-6143 113:4<279 2000 113 418 https://doi.org/10.1007/s004180000131 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000131 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haila Siru Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut NATIONALLICENCE 700 1- Saarialho-Kere Ulpu Department of Dermatology, 00029 Helsinki University Central Hospital, Finland aut NATIONALLICENCE 700 1- Karjalainen-Lindsberg Marja-Liisa Department of Pathology, Haartman Institute, 00014 University of Helsinki, Finland aut NATIONALLICENCE 700 1- Lohi Hannes Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut NATIONALLICENCE 700 1- Airola Kristiina Department of Dermatology, 00029 Helsinki University Central Hospital, Finland aut NATIONALLICENCE 700 1- Holmberg Christer Hospital for Children and Adolescents, 00029 Helsinki University Central Hospital, Finland aut NATIONALLICENCE 700 1- Hästbacka Johanna Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut NATIONALLICENCE 700 1- Kere Juha Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut NATIONALLICENCE 700 1- Höglund Pia Department of Medical Genetics, Haartman Institute, P.O.Box 21 (Haartmaninkatu 3), 00014 University of Helsinki, Finland aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 279-286 0948-6143 113:4<279 2000 113 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer