caa a22 4500 475774868 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004180000130 doi (NATIONALLICENCE)springer-10.1007/s004180000130 Expression, characterization, and localization of Rab26, a low molecular weight GTP-binding protein, in the rat parotid gland [Elektronische Daten] [Sumio Yoshie, Akane Imai, Tomoko Nashida, Hiromi Shimomura] We investigated the expression of the genes encoding Rab proteins, low molecular weight GTP-binding proteins, in the rat parotid gland by the use of reverse transcription-polymerase chain reaction, and detected cDNAs of Rab3D, Rab4, and Rab26. We further examined the characteristics and localization of Rab26 by western blotting and light and electron microscopic immunocytochemistry. Western blotting using an antibody against the Rab26-specific, C-terminal peptide detected the His-tagged Rab26 protein as a single 27-kDa band. This band also displayed binding to [α-32P]GTP. The fraction containing secretory granule membranes in an acinar cell homogenate was immunostained with the antibody. Supporting this, the immunocytochemical reaction for Rab26 was localized immediately around secretory granules in the acinar cells. The immunostaining disappeared from the acinar cells after treatment of rats with isoproterenol. These findings suggest that Rab26 participates in the regulated secretion of granules and functionally belongs to the Rab3 group. Springer-Verlag, 2000 Yoshie Sumio Department of Oral Anatomy, Nippon Dental University School of Dentistry, 1-8, Hamauracho, Niigata 951-8580, Japan aut Imai Akane Department of Oral Biochemistry, Nippon Dental University School of Dentistry, Niigata, Japan aut Nashida Tomoko Department of Oral Biochemistry, Nippon Dental University School of Dentistry, Niigata, Japan aut Shimomura Hiromi Department of Oral Biochemistry, Nippon Dental University School of Dentistry, Niigata, Japan aut Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 259-263 0948-6143 113:4<259 2000 113 418 https://doi.org/10.1007/s004180000130 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000130 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoshie Sumio Department of Oral Anatomy, Nippon Dental University School of Dentistry, 1-8, Hamauracho, Niigata 951-8580, Japan aut NATIONALLICENCE 700 1- Imai Akane Department of Oral Biochemistry, Nippon Dental University School of Dentistry, Niigata, Japan aut NATIONALLICENCE 700 1- Nashida Tomoko Department of Oral Biochemistry, Nippon Dental University School of Dentistry, Niigata, Japan aut NATIONALLICENCE 700 1- Shimomura Hiromi Department of Oral Biochemistry, Nippon Dental University School of Dentistry, Niigata, Japan aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 113/4(2000-04-01), 259-263 0948-6143 113:4<259 2000 113 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer