caa a22 4500 475774876 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004180000149 doi (NATIONALLICENCE)springer-10.1007/s004180000149 A method for preparing 2- to 50-µm-thick fresh-frozen sections of large samples and undecalcified hard tissues [Elektronische Daten] [Tadafumi Kawamoto, Masaharu Shimizu] Abstract.: This article describes a method for preparing 2- to 50-µm-thick fresh-frozen sections from large samples and completely calcified tissue samples. In order to perform the more routine work involved, a tungsten carbide disposable blade was installed to a heavy-duty sledge cryomicrotome. An entire 10-day-old rat and bone and tooth samples from a 7-month-old rat were rapidly frozen. The frozen samples were attached to the cryomicrotome stage. The cutting surface of the samples was covered with a polyvinylidene chloride film coated with synthetic rubber cement and cut at -25°C. The soft tissues and the hard tissues were satisfactorily preserved and all tissue cells were easily identifiable. Enzymatic activity in the fresh sections was much stronger than that in chemically fixed and/or decalcified sections. The sections permitted histological and histochemical studies without trouble. In addition, the sections can be used for multiple experiments such as immunohistochemistry, in situ hybridization, and electron microprobe X-ray microanalysis. This method can be used with conventional cryomicrotome equipment. Springer-Verlag, 2000 Fresh-frozen section Whole-body section Hard-tissue section Enzyme histochemistry Bone Tooth nationallicence Kawamoto Tadafumi Department of Biochemistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Japan aut Shimizu Masaharu Department of Biochemistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Japan aut https://doi.org/10.1007/s004180000149 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000149 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawamoto Tadafumi Department of Biochemistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Japan aut NATIONALLICENCE 700 1- Shimizu Masaharu Department of Biochemistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Japan aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer