caa a22 4500 475774914 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004180000148 doi (NATIONALLICENCE)springer-10.1007/s004180000148 The apical membrane of intestinal brush cells possesses a specialised, but species-specific, composition of glycoconjugates - on-section and in vivo lectin labelling in rats, guinea-pigs and mice [Elektronische Daten] [Andreas Gebert, Kais Al-Samir, Kerstin Werner, Susanne Fassbender, Anke Gebhard] Abstract.: Brush cells are specialised epithelial cells that are assumed to represent chemoreceptors of the digestive tract. They comprise a small population of the epithelial cells lining the intestine, possess a unique ultrastructure and, in many aspects, resemble the receptor cells of taste buds. To characterise glycoconjugates possibly involved in a sensory function, we investigated brush cells in the small intestine of three species using lectin histochemistry in confocal light and thin-section electron microscopy. Brush cells of rats were selectively labelled by the sialic acid-specific lectin Maackia amurensis agglutinin, those of guinea-pigs by the d-galactose-specific lectin Bandeiraea simplicifolia agglutinin, isolectin B4 and those of mice by the l-fucose-specific lectin Ulex europaeus agglutinin lectinI. Lectin binding sites were consistently located in the glycocalyx of the apical membrane and in that of cytoplasmic vesicles. In vivo lectin labelling revealed that the glycoconjugates of the apical membrane are accessible under physiological conditions, that brush cells do not endocytose and that they probably possess a high membrane turnover rate. The results show that specialisations exist in the composition of glycoconjugates forming the glycocalyx of brush cells in all species investigated. The presence of brush cell-specific glycoconjugates would be in accordance with the current hypothesis of a receptive function of brush cells. Differences in the specific glycosylation patterns among rats, guinea-pigs and mice indicate that species-specific adaptations exist. Springer-Verlag, 2000 Brush cell Lectin Glycocalyx Receptor Rat Guinea-pig Mouse nationallicence Gebert Andreas Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Al-Samir Kais Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Werner Kerstin Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Fassbender Susanne Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Gebhard Anke Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut https://doi.org/10.1007/s004180000148 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000148 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gebert Andreas Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 700 1- Al-Samir Kais Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 700 1- Werner Kerstin Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 700 1- Fassbender Susanne Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut NATIONALLICENCE 700 1- Gebhard Anke Abteilung Anatomie 2, Medizinische Hochschule Hannover, 30623 Hannover, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer