caa a22 4500 475774922 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004180000153 doi (NATIONALLICENCE)springer-10.1007/s004180000153 Expression of the cholinergic gene locus in pulmonary arterial endothelial cells [Elektronische Daten] [Rainer Viktor Haberberger, Martin Bodenbenner, Wolfgang Kummer] Abstract.: In the pulmonary vasculature of man, pig and guinea-pig, acetylcholine (ACh) exerts a relaxant effect by interacting with muscarinic receptors located on endothelial cells. The present experiments were designed to detect the endogenous source of ACh in the pulmonary vasculature. For this purpose, we investigated whether pulmonary artery endothelial cells contain elements of the "cholinergic gene locus", the ACh synthesising enzyme choline acetyltransferase (ChAT) and the vesicular ACh transporter (VAChT). ChAT mRNA was detected by means of reverse transcription polymerase chain reaction (RT-PCR) in endothelial cells of porcine pulmonary arteries freshly isolated and after 7days in culture. ChAT protein was demonstrated in endothelial cells in vitro and in situ. ChAT immunoreactivity was present in endothelial cells freshly isolated and after 2, 4, 7, and 9days in culture. Tissue sections from extra- and intraparenchymal pulmonary arteries of man, pig and guinea-pig expressed a mosaic pattern of ChAT-positive and -negative endothelial cells. VAChT mRNA was detected by RT-PCR in rat pulmonary artery and in endothelial cells isolated from human and porcine pulmonary trunk. The detection of VAChT and ChAT mRNA and the demonstration of ChAT protein in vitro and in situ suggest that the endothelium is an endogenous source of ACh in the pulmonary vasculature. Springer-Verlag, 2000 Choline acetyltransferase Vesicular acetylcholine transporter Immunohistochemistry Human Porcine nationallicence Haberberger Rainer Viktor Institute for Anatomy and Cell Biology, Justus-Liebig-University, Aulweg 123, 35385 Giessen, Germany aut Bodenbenner Martin Institute for Anatomy and Cell Biology, Justus-Liebig-University, Aulweg 123, 35385 Giessen, Germany aut Kummer Wolfgang Institute for Anatomy and Cell Biology, Justus-Liebig-University, Aulweg 123, 35385 Giessen, Germany aut https://doi.org/10.1007/s004180000153 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180000153 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haberberger Rainer Viktor Institute for Anatomy and Cell Biology, Justus-Liebig-University, Aulweg 123, 35385 Giessen, Germany aut NATIONALLICENCE 700 1- Bodenbenner Martin Institute for Anatomy and Cell Biology, Justus-Liebig-University, Aulweg 123, 35385 Giessen, Germany aut NATIONALLICENCE 700 1- Kummer Wolfgang Institute for Anatomy and Cell Biology, Justus-Liebig-University, Aulweg 123, 35385 Giessen, Germany aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer