caa a22 4500 475774973 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s004180050016 doi (NATIONALLICENCE)springer-10.1007/s004180050016 Lineage-related sensitivity to apoptosis in human tumor cells undergoing hyperthermia [Elektronische Daten] [E. Falcieri, F. Luchetti, S. Burattini, B. Canonico, S. Santi, S. Papa] Abstract : In this study the role of hyperthermia as an apoptotic trigger was analyzed in four human tumor cell lines: HL60, U937, DOHH2, and K562. These cell lines were chosen because of their well known and different expression of bcl-2 and bcr-abl genes, the expression of which is known to be an antiapoptotic condition. HL60 and U937 cells were strongly susceptible to heat exposure, while DOHH2 cells were weakly sensitive and K562 cells were resistant, thus suggesting a possible gene involvement in this type of programmed cell death. The mechanisms underlying this apoptosis were investigated by flow cytometry, agarose gel electrophoresis, and light and electron microscopy. A subdiploid peak and DNA laddering, both of which are parameters specifically correlated to programmed cell death, were present in HL60 and U937 and, even if less evident, in DOHH2 cells undergoing hyperthermic treatment, and were absent in K562 cells. In addition, DNA single-strand cleavage was revealed by in situ nick translation, observed by confocal microscopy. Morphological analysis confirmed these results and revealed the typical chromatin changes, followed by the appearance of micronuclei and apoptotic bodies. Springer-Verlag Berlin Heidelberg, 2000 Falcieri E. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut Luchetti F. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut Burattini S. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut Canonico B. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut Santi S. Istituto di Citomorfologia Normale e Patologica, CNR, Istituti Ortopedici Rizzoli, Bologna, Italy, IT aut Papa S. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut https://doi.org/10.1007/s004180050016 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050016 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Falcieri E. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut NATIONALLICENCE 700 1- Luchetti F. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut NATIONALLICENCE 700 1- Burattini S. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut NATIONALLICENCE 700 1- Canonico B. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut NATIONALLICENCE 700 1- Santi S. Istituto di Citomorfologia Normale e Patologica, CNR, Istituti Ortopedici Rizzoli, Bologna, Italy, IT aut NATIONALLICENCE 700 1- Papa S. Centro di Citometria e Citomorfologia, Università di Urbino, Campus Scientifico, Località Crocicchia, 61029 Urbino, Italy e-mail: e.falcieri@uniurb.it Tel.: +39-722-304284/267, Fax: +39-722-304226, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer