caa a22 4500 475775007 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s004180050013 doi (NATIONALLICENCE)springer-10.1007/s004180050013 Induction of the blood-brain barrier marker neurothelin/HT7 in endothelial cells by a variety of tumors in chick embryos [Elektronische Daten] [M. Papoutsi, H. Kurz, C. Schächtele, D. Marmé, B. Christ, F. Pröls, J. Wilting] Abstract : Neurothelin/HT7, a transmembrane glycoprotein of the immunoglobulin superfamily, is a marker of blood-brain barrier (BBB)-forming endothelial cells. We have studied the expression of neurothelin in tumors grown on the chorioallantoic membrane (CAM) of chick embryos. We inoculated each 3-5×106 rat C6 glioma, rat 10AS pancreatic carcinoma, human A375 melanoma, and human mammary duct adenoma cells on the CAM of 10-day-old chick embryos. The tumors were harvested on day 17. All four tumor cell lines formed solid tumors which were supplied by vessels of CAM origin. Foci of bleeding were regularly observed within the tumors. All four tumors induced the expression of neurothelin/HT7 (but not of glucose transporter-1) in tumor endothelial cells, whereas expression in adjacent endothelial cells of normal CAM did not occur. Confocal laser scanning microscopy revealed that the pattern of neurothelin expression in tumor endothelial cells was different from that in normal central nervous system (CNS) endothelium, but the relative molecular weight of neurothelin, studied by western blot analysis, was the same in brain and in tumors. It has been shown that, with increasing malignancy, vessels of CNS tumors lose their morphological characteristics, and BBB markers such as the glucose transporter-1 are downregulated. Our results show that, in contrast, the BBB marker, neurothelin, is expressed de novo in tumor endothelial cells. Potential common functions of neurothelin in endothelial cells of the CNS and tumors are discussed. Springer-Verlag Berlin Heidelberg, 2000 Papoutsi M. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut Kurz H. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut Schächtele C. Institut für Molekulare Medizin und Naturstofforschung, Klinik für Tumorbiologie an der Albert-Ludwigs-Universität Freiburg, 79011 Freiburg, Germany, DE aut Marmé D. Institut für Molekulare Medizin und Naturstofforschung, Klinik für Tumorbiologie an der Albert-Ludwigs-Universität Freiburg, 79011 Freiburg, Germany, DE aut Christ B. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut Pröls F. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut Wilting J. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut https://doi.org/10.1007/s004180050013 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050013 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Papoutsi M. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut NATIONALLICENCE 700 1- Kurz H. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut NATIONALLICENCE 700 1- Schächtele C. Institut für Molekulare Medizin und Naturstofforschung, Klinik für Tumorbiologie an der Albert-Ludwigs-Universität Freiburg, 79011 Freiburg, Germany, DE aut NATIONALLICENCE 700 1- Marmé D. Institut für Molekulare Medizin und Naturstofforschung, Klinik für Tumorbiologie an der Albert-Ludwigs-Universität Freiburg, 79011 Freiburg, Germany, DE aut NATIONALLICENCE 700 1- Christ B. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut NATIONALLICENCE 700 1- Pröls F. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut NATIONALLICENCE 700 1- Wilting J. Anatomisches Institut der Albert-Ludwigs-Universität Freiburg, Albertstrasse 17, 79104 Freiburg, Germany e-mail: wilting@uni-freiburg.de Tel.: +49-761-2035087, Fax: +49-761-2035091, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer