caa a22 4500 475775015 CHVBK 20180406123628.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s004180050011 doi (NATIONALLICENCE)springer-10.1007/s004180050011 Coexistence of gland mucous cell-type mucin and lysozyme in gastric gland mucous cells [Elektronische Daten] [E. Hidaka, H. Ota, T. Katsuyama, J. Nakayama, M. Momose, H. Hidaka, K. Ishii, F. Murata, S. Tsuyama, M. Kurihara, K. Ishihara, K. Hotta] Abstract : Class III mucin, identified by paradoxical concanavalin A staining, is confined to gastric gland mucous cells and is an essential component of the gastric surface mucous gel layer. The pretreatment required has hampered the application of this method to electron microscopic studies. Antibody HIK1083 reacts selectively with class III mucins. The present study was undertaken to explore, electron microscopically, the immunoreactivity of the human stomach to HIK1083. We examined normal mucosa from resected human stomachs (five cases; formalin-fixed, paraffin-embedded) and gastric biopsy specimens from patients with early gastric cancer [nine cases; glutaraldehyde- and osmium-fixed, epoxy-embedded (seven cases) and half-strength Karnovsky's solution-fixed, Lowicryl K4M-embedded (two cases)]. Immunostaining with HIK1083 and anti-lysozyme antibody was examined under light and electron microscopes. Gland mucous cells were labeled with HIK1083, and lysozyme was detected in some gland mucous cells and surface mucous cells. Electron microscopically, the secretory granules of gland mucous cells contained a single electron-dense core. HIK1083-positive mucins and lysozyme coexisted in the secretory granules of gastric gland mucous cells. HIK1083-reactive mucins and lysozyme were distributed in the matrix and in the dense core of these secretory granules, respectively. HIK1083 can be used for electron immunohistochemistry. Springer-Verlag Berlin Heidelberg, 2000 Hidaka E. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut Ota H. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut Katsuyama T. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut Nakayama J. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut Momose M. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut Hidaka H. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut Ishii K. Departments of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan, JP aut Murata F. Department of Anatomy, Kagoshima University School of Medicine, 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890-8520, Japan, JP aut Tsuyama S. Department of Anatomy, Kagoshima University School of Medicine, 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890-8520, Japan, JP aut Kurihara M. Isehara Research Laboratory, Kanto Chemical Company, 21 Suzukawa, Isehara, Kanagawa 259-1146, Japan, JP aut Ishihara K. Department of Chemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan, JP aut Hotta K. Department of Biochemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan, JP aut https://doi.org/10.1007/s004180050011 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004180050011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hidaka E. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut NATIONALLICENCE 700 1- Ota H. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut NATIONALLICENCE 700 1- Katsuyama T. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut NATIONALLICENCE 700 1- Nakayama J. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut NATIONALLICENCE 700 1- Momose M. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut NATIONALLICENCE 700 1- Hidaka H. Central Clinical Laboratories, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan e-mail: hota@hsp.md.shinshu-u.ac.jp Tel.: +81-263-372805, Fax: +81-263-345316, JP aut NATIONALLICENCE 700 1- Ishii K. Departments of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan, JP aut NATIONALLICENCE 700 1- Murata F. Department of Anatomy, Kagoshima University School of Medicine, 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890-8520, Japan, JP aut NATIONALLICENCE 700 1- Tsuyama S. Department of Anatomy, Kagoshima University School of Medicine, 8-35-1 Sakuragaoka, Kagoshima, Kagoshima 890-8520, Japan, JP aut NATIONALLICENCE 700 1- Kurihara M. Isehara Research Laboratory, Kanto Chemical Company, 21 Suzukawa, Isehara, Kanagawa 259-1146, Japan, JP aut NATIONALLICENCE 700 1- Ishihara K. Department of Chemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan, JP aut NATIONALLICENCE 700 1- Hotta K. Department of Biochemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer