caa a22 4500 475775066 CHVBK 20180406123629.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s00418-004-0704-y doi (NATIONALLICENCE)springer-10.1007/s00418-004-0704-y Sequence of IGF-I, IGF-II, and HGF expression in regenerating skeletal muscle [Elektronische Daten] [Shinichiro Hayashi, Hisashi Aso, Kouichi Watanabe, Hidetoshi Nara, Michael Rose, Shyuichi Ohwada, Takahiro Yamaguchi] Various cytokines are thought to play a role in muscle regeneration, however, the interaction and mechanisms of action of these cytokines remains largely unknown. In this study, we investigated the role of HGF, IGF-I, and IGF-II during myogenesis using the regeneration model of skeletal muscle as well as myoblast culture. RT-PCR analysis revealed that HGF and IGF-I expressions were markedly upregulated, in regenerating muscle. In contrast, there was no significant difference in IGF-II expression between normal and regenerating muscle. Immunohistochemical analysis demonstrated that HGF was expressed mostly by myocytes during the early stages of muscle regeneration. Additionally, HGF inhibited the formation of myotubes by myoblasts, but promoted cellular proliferation. Otherwise, IGF-I and IGF-II were expressed by myocytes through the early to middle stages of muscle regeneration. The addition of HGF to myoblast growing in vitro significantly increased the number of cells. These findings indicate that these three cytokines have pleiotropic effects in regenerating skeletal muscle. Springer-Verlag, 2004 Bovine nationallicence Skeletal muscle nationallicence HGF nationallicence IGF-I nationallicence IGF-II nationallicence Hayashi Shinichiro Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut Aso Hisashi Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut Watanabe Kouichi Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut Nara Hidetoshi Department of Immunology, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, 990-9585, Yamagata, Japan aut Rose Michael Institute of Rural Sciences, University of Wales, SY233AL, Aberystwyth, UK aut Ohwada Shyuichi Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut Yamaguchi Takahiro Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut Histochemistry and Cell Biology Springer-Verlag 122/5(2000-11-01), 427-434 0948-6143 122:5<427 2000 122 418 https://doi.org/10.1007/s00418-004-0704-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00418-004-0704-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hayashi Shinichiro Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Aso Hisashi Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Watanabe Kouichi Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Nara Hidetoshi Department of Immunology, Yamagata University School of Medicine, 2-2-2 Iida-Nishi, 990-9585, Yamagata, Japan aut NATIONALLICENCE 700 1- Rose Michael Institute of Rural Sciences, University of Wales, SY233AL, Aberystwyth, UK aut NATIONALLICENCE 700 1- Ohwada Shyuichi Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Yamaguchi Takahiro Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 122/5(2000-11-01), 427-434 0948-6143 122:5<427 2000 122 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer