caa a22 4500 475775082 CHVBK 20180406123629.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s00418-004-0717-6 doi (NATIONALLICENCE)springer-10.1007/s00418-004-0717-6 Shear stress-induced upregulation of connexin43 expression in endothelial cells on upstream surfaces of rat cardiac valves [Elektronische Daten] [Tetsuichiro Inai, Michael Mancuso, Donald McDonald, Junichi Kobayashi, Kyoko Nakamura, Yosaburo Shibata] Endothelial expression of the gap junction proteins, connexin (Cx)37, Cx40, and Cx43, varies within the vascular network. While previous studies suggest that shear stress may upregulate Cx43, it is not well understood if shear stress affects the expression of all endothelial connexins and to what extent. Endothelial cells on the upstream and downstream surfaces of cardiac valves are subjected to considerably different intensities of shear stress. We therefore reasoned that we could determine the extent hemodynamic forces affect the expression of Cx37, Cx40, and Cx43 by comparing their immunohistochemical distribution on the upstream and downstream surfaces of rat cardiac valves. We found 70- to 200-fold greater expression of Cx43 in the endothelial cells on the upstream than on the downstream surfaces. However, Cx37 was expressed almost equally in the endothelial cells on upstream and downstream surfaces, and Cx40, a major connexin in most vascular endothelial cells, was not detected on either surface. In addition to the heterogeneity in Cx43 expression, endothelial cells on the upstream surface were 35% to 65% smaller than those on the corresponding downstream surface. These results suggest that shear stress may affect endothelial cell size and Cx43 expression but not Cx37 expression. Springer-Verlag, 2004 Connexin nationallicence Endothelial cell nationallicence Cardiac valve nationallicence Shear stress nationallicence Size nationallicence Inai Tetsuichiro Department of Developmental Molecular Anatomy, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, 812-8582, Fukuoka, Japan aut Mancuso Michael Cardiovascular Research Institute, Comprehensive Cancer Center, and Department of Anatomy, University of California, CA94143, San Francisco, USA aut McDonald Donald Cardiovascular Research Institute, Comprehensive Cancer Center, and Department of Anatomy, University of California, CA94143, San Francisco, USA aut Kobayashi Junichi Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Nakamura Kyoko Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut Shibata Yosaburo Department of Developmental Molecular Anatomy, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, 812-8582, Fukuoka, Japan aut Histochemistry and Cell Biology Springer-Verlag 122/5(2000-11-01), 477-483 0948-6143 122:5<477 2000 122 418 https://doi.org/10.1007/s00418-004-0717-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00418-004-0717-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inai Tetsuichiro Department of Developmental Molecular Anatomy, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, 812-8582, Fukuoka, Japan aut NATIONALLICENCE 700 1- Mancuso Michael Cardiovascular Research Institute, Comprehensive Cancer Center, and Department of Anatomy, University of California, CA94143, San Francisco, USA aut NATIONALLICENCE 700 1- McDonald Donald Cardiovascular Research Institute, Comprehensive Cancer Center, and Department of Anatomy, University of California, CA94143, San Francisco, USA aut NATIONALLICENCE 700 1- Kobayashi Junichi Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nakamura Kyoko Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan aut NATIONALLICENCE 700 1- Shibata Yosaburo Department of Developmental Molecular Anatomy, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, 812-8582, Fukuoka, Japan aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 122/5(2000-11-01), 477-483 0948-6143 122:5<477 2000 122 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer