caa a22 4500 475775090 CHVBK 20180406123629.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s00418-004-0707-8 doi (NATIONALLICENCE)springer-10.1007/s00418-004-0707-8 Gender dimorphism influences extracellular matrix expression and regeneration of muscular tissue in mdx dystrophic mice [Elektronische Daten] [Maria Salimena, Jussara Lagrota-Candido, Thereza Quírico-Santos] Mdx mouse, the animal model of Duchenne muscular dystrophy, lacks dystrophin and develops an X-linked recessive inflammatory myopathy characterized by degeneration of skeletal muscle fibers and connective tissue replacement. The present work aimed to assess whether gender dimorphism in mdx mice would influence skeletal muscle pathology at ages corresponding to main histological changes in the microenvironment of muscular tissue: myonecrosis, regeneration, and fibrosis. At the height of myonecrosis (6weeks postnatal), skeletal muscles of male mdx mice showed increased sarcolemmal permeability, numerous inflammatory foci, and marked deposition of the extracellular matrix components (ECM) typeI collagen and laminin. In contrast, age-matched mdx females showed mild ECM deposition, discrete myonecrosis, but increased numbers of regenerating fibers expressing the satellite cell marker NCAM. In contrast ovariectomized mdx females showed decreased numbers of regenerating fibers. Older (24 and 48weeks postnatal) mdx females showed extensive fibrosis with increased sarcolemmal permeability and marked deposition of ECM components than corresponding males. These results suggest a role for female hormones in the control of myonecrosis probably by promoting regeneration of muscular tissue and mitigating inflammation especially at ages under the critical influence of sex hormones. Springer-Verlag, 2004 Muscular dystrophy nationallicence Extracellular matrix nationallicence Mdx nationallicence Gender dimorphism nationallicence Salimena Maria Department of Immunobiology, Institute of Biology, Fluminense Federal University, Rio de Janeiro, Brazil aut Lagrota-Candido Jussara Department of Immunobiology, Institute of Biology, Fluminense Federal University, Rio de Janeiro, Brazil aut Quírico-Santos Thereza Department of Cellular and Molecular Biology, Institute of Biology, Fluminense Federal University, 94910-130, Rio de Janeiro, Brazil aut Histochemistry and Cell Biology Springer-Verlag 122/5(2000-11-01), 435-444 0948-6143 122:5<435 2000 122 418 https://doi.org/10.1007/s00418-004-0707-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00418-004-0707-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salimena Maria Department of Immunobiology, Institute of Biology, Fluminense Federal University, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Lagrota-Candido Jussara Department of Immunobiology, Institute of Biology, Fluminense Federal University, Rio de Janeiro, Brazil aut NATIONALLICENCE 700 1- Quírico-Santos Thereza Department of Cellular and Molecular Biology, Institute of Biology, Fluminense Federal University, 94910-130, Rio de Janeiro, Brazil aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 122/5(2000-11-01), 435-444 0948-6143 122:5<435 2000 122 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer