caa a22 4500 475775120 CHVBK 20180406123629.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s00418-004-0708-7 doi (NATIONALLICENCE)springer-10.1007/s00418-004-0708-7 Localization of endothelin receptors in bleomycin-induced pulmonary fibrosis in the rat [Elektronische Daten] [Martina Wendel, Anna Petzold, Roland Koslowski, Michael Kasper, Antje Augstein, Lilla Knels, Jörg-Uwe Bleyl, Thea Koch] Pulmonary fibrosis is characterized by excessive extracellular matrix deposition with concomitant loss of gas exchange units, and endothelin-1 (ET-1) has been implicated in its pathogenesis. Increased levels of ET-1 from tissues and bronchoalveolar lavage have been reported in patients with pulmonary fibrosis and in animal models after intratracheal bleomycin. We characterized the cellular distribution of alveolar ET receptors by immunohistochemistry in bleomycin-induced pulmonary fibrosis in the rat and determined the regulation by bleomycin of ET receptor mRNA expression in isolated alveolar macrophages and rat lung fibroblasts. We found significant increases in the numbers of fibroblasts and macrophages at day7 compared to day28 and control animals. ETB receptor immunoreactivity was observed on fibroblasts and invading monocytes. Isolated fibroblasts expressed both ETA and ETB receptor mRNA, and ETA receptor mRNA was upregulated by bleomycin. Isolated resident alveolar macrophages expressed neither ETA nor ETB receptor mRNA which were also not induced by bleomycin. We conclude that, while ETB receptor stimulation of fibroblasts and monocytes recruited during bleomycin-induced lung injury exerts antagonistic effects on fibroblast collagen synthesis, the observed increase in the number of fibroblasts in vivo and upregulation of fibroblast ETA receptor mRNA by bleomycin in vitro point to a predominance of the profibrotic effects of ET receptor engagement. Springer-Verlag, 2004 Endothelin-A receptor nationallicence Endothelin-B receptor nationallicence Rat nationallicence Pulmonary fibrosis nationallicence Immunohistochemistry nationallicence Quantitative PCR nationallicence Wendel Martina Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut Petzold Anna Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut Koslowski Roland Department of Physiological Chemistry, Medical Faculty of the Technical University of Dresden, Fiedlerstrasse42, 01307, Dresden, Germany aut Kasper Michael Department of Anatomy, Medical Faculty of the Technical University of Dresden, Fiedlerstrasse42, 01307, Dresden, Germany aut Augstein Antje Department of Cardiology, Medical Clinics II, Medical Faculty of the Technical University of Dresden, Fiedlerstrasse42, 01307, Dresden, Germany aut Knels Lilla Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut Bleyl Jörg-Uwe Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut Koch Thea Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut Histochemistry and Cell Biology Springer-Verlag 122/5(2000-11-01), 507-517 0948-6143 122:5<507 2000 122 418 https://doi.org/10.1007/s00418-004-0708-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00418-004-0708-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wendel Martina Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Petzold Anna Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Koslowski Roland Department of Physiological Chemistry, Medical Faculty of the Technical University of Dresden, Fiedlerstrasse42, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Kasper Michael Department of Anatomy, Medical Faculty of the Technical University of Dresden, Fiedlerstrasse42, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Augstein Antje Department of Cardiology, Medical Clinics II, Medical Faculty of the Technical University of Dresden, Fiedlerstrasse42, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Knels Lilla Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Bleyl Jörg-Uwe Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut NATIONALLICENCE 700 1- Koch Thea Department of Anesthesiology, University Hospital Dresden, Fetscherstrasse74, 01307, Dresden, Germany aut NATIONALLICENCE 773 0- Histochemistry and Cell Biology Springer-Verlag 122/5(2000-11-01), 507-517 0948-6143 122:5<507 2000 122 418 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer