caa a22 4500 47577521X CHVBK 20180406123629.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1023/A:1007239017018 doi (NATIONALLICENCE)springer-10.1023/A:1007239017018 The time course of responses to intratracheally instilled toxic Stachybotrys chartarum spores in rats [Elektronische Daten] [Carol Rao, Harriet Burge, Joseph Brain] Stachybotrys chartarum is a fungal species that can produce mycotoxins, specifically trichothecenes. Exposures in the indoor environment have reportedly induced neurogenic symptoms in adults and hemosiderosis in infants. However, little evidence has linked measured exposures to any fungal agent with any health outcome. We present here a study that focuses on quantitatively assessing the health risks from fungal toxin exposure. Male, 10 week old Charles River-Dawley rats were intratracheally instilled with approximately 9.6 million Stachybotrys chartarum spores in a saline suspension. The lungs were lavaged 0 h (i.e., immediately post-instillation), 6, 24 or 72 h after instillation. Biochemical indicators (albumin, myeloperoxidase, lactic dehydrogenase, hemoglobin) and leukocyte differentials in the bronchoalveolar lavage fluid and weight change were measured. We have demonstrated that a single, acute pulmonary exposure to a large quantity of Stachybotrys chartarum spores by intratracheal instillation causes severe injury detectable by bronchoalveolar lavage. The primary effect appears to be cytotoxicity and inflammation with hemorrhage. There is a measurable effect as early as 6 h after instillation, which may be attributable to mycotoxins in the fungal spores. The time course of responses supports early release of some toxins, with the most severe effects occurring between 6 and 24 h following exposure. By 72 h, recovery has begun, although macrophage concentrations remained elevated. Kluwer Academic Publishers, 2000 Stachybotrys chartarum nationallicence bronchoalveolar lavage nationallicence fungi nationallicence mycotoxins nationallicence lactic dehydrogenase nationallicence hemorrhage nationallicence Rao Carol Department of Environmental Health, Harvard School of Public Health, 02115, Boston, MA, USA aut Burge Harriet Department of Environmental Health, Harvard School of Public Health, 02115, Boston, MA, USA aut Brain Joseph Department of Environmental Health, Harvard School of Public Health, 02115, Boston, MA, USA aut Mycopathologia Kluwer Academic Publishers 149/1(2000-08-01), 27-34 0301-486X 149:1<27 2000 149 11046 https://doi.org/10.1023/A:1007239017018 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007239017018 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rao Carol Department of Environmental Health, Harvard School of Public Health, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Burge Harriet Department of Environmental Health, Harvard School of Public Health, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Brain Joseph Department of Environmental Health, Harvard School of Public Health, 02115, Boston, MA, USA aut NATIONALLICENCE 773 0- Mycopathologia Kluwer Academic Publishers 149/1(2000-08-01), 27-34 0301-486X 149:1<27 2000 149 11046 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer